Cells were imaged using a Nikon TE-2000E inverted microscope with a 603 1.49 NA TIRF objective, Andor Revolution XD spinning disk confocal system, and 488 and 568 nm solid-state lasers. Cells were imaged in Opti-MEM or Leibowitz's L15 medium (Gibco), 5% FBS, at 37°C. Time lapses were acquired using an Andor iXon+ EM-CCD camera using Andor IQ. Original 16-bit tif files acquired directly from the camera were used for image analysis.
Xd spinning disk confocal system
Manufactured by Oxford Instruments
The XD spinning disk confocal system is a high-performance imaging solution designed for advanced biological research. It utilizes a spinning disk to provide fast, high-resolution imaging of live cell samples. The system features a modular design and can be customized to meet the specific needs of researchers.
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2 protocols using xd spinning disk confocal system
1
Live-cell Imaging of Cellular Dynamics
2
Live-cell Imaging of Cellular Dynamics
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Cells were imaged using a Nikon TE-2000E inverted microscope with a 603 1.49 NA TIRF objective, Andor Revolution XD spinning disk confocal system, and 488 and 568 nm solid-state lasers. Cells were imaged in Opti-MEM or Leibowitz's L15 medium (Gibco), 5% FBS, at 37°C. Time lapses were acquired using an Andor iXon+ EM-CCD camera using Andor IQ. Original 16-bit tif files acquired directly from the camera were used for image analysis.
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