Full-length wild-type S. aureus gyrase subunits (GyrA and GyrB, used for enzymological studies), as well as the wild-type gyrase core fusion truncate (GyrB27-A56) and a fusion truncate containing a GyrAY123F mutation (used for structural studies) were expressed and purified as described previously.25 (link)Negatively supercoiled pBR322 DNA was prepared from Escherichia coli using a Plasmid Mega Kit (Qiagen) as described by the manufacturer. Positively supercoiled pBR322 DNA was prepared by treating negatively supercoiled molecules with recombinant Archaeoglobus fulgidus reverse gyrase.49 (link)–50 (link) The number of positive supercoils induced by this process is comparable to the number of negative supercoils in the original pBR322 preparations.49 (link) In the experiments that compared negatively and positively supercoiled DNA, the negatively supercoiled plasmid preparations were processed identically to the positively supercoiled molecules except that reaction mixtures did not contain reverse gyrase. Relaxed pBR322 plasmid DNA was generated by treating negatively supercoiled pBR322 with calf thymus topoisomerase I (Invitrogen) and purified as described previously.27 (link)Gepotidacin was provided by GlaxoSmithKline. Moxifloxacin was obtained from LKT Laboratories. Gepotidacin and Moxifloxacin were stored at 4 °C as 20 mM stock solutions in 100% dimethyl sulfoxide.
Moxifloxacin
Manufactured by LKT Laboratories
Sourced in United States
Moxifloxacin is a broad-spectrum antibiotic agent used for the identification and quantification of moxifloxacin in various sample matrices. It functions as an analytical standard for use in research and quality control applications.
Automatically generated - may contain errors
Lab products found in correlation
Ampicillin, by Fujifilm (3 mentions)
Plasmid mega kit, by Qiagen (1 mentions)
Gepotidacin, by GlaxoSmithKline (1 mentions)
Calf thymus topoisomerase 1, by Thermo Fisher Scientific (1 mentions)
Tetracycline, by Fujifilm (1 mentions)
Amikacin, by Fujifilm (1 mentions)
Carbenicillin, by Fujifilm (1 mentions)
Chloramphenicol, by Fujifilm (1 mentions)
Ciprofloxacin, by Fujifilm (1 mentions)
Norfloxacin, by Fujifilm (1 mentions)
Levofloxacin, by LKT Laboratories (1 mentions)
Sitafloxacin, by Daiichi Sankyo (1 mentions)
Hygromycin, by Fujifilm (1 mentions)
Kanamycin, by Fujifilm (1 mentions)
Dna ligation kit, by Takara Bio (1 mentions)
Mighty mix, by Takara Bio (1 mentions)
In fusion hd cloning kit, by Takara Bio (1 mentions)
Restriction enzyme, by New England Biolabs (1 mentions)
Ofloxacin, by LKT Laboratories (1 mentions)
7 protocols using moxifloxacin
1
Purification and Supercoiling of pBR322 DNA
2
Antimicrobial Agents Procurement Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Amikacin, ampicillin, carbenicillin, chloramphenicol, ciprofloxacin, norfloxacin, and tetracycline were purchased from Wako Pure Chemicals Industries, Ltd (Osaka, Japan). Moxifloxacin and levofloxacin were purchased from LKT Laboratories, Inc. (St. Paul, MN, USA). Imipenem/cilastatin was purchased from Sandoz K.K. (Tokyo, Japan).
3
Inactivation of S. marcescens by Antibiotics and Heat
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Overnight cultures of S. marcescens were normalized to
OD600 = 2. One ml of normalized culture was treated
with 50 μg/ml moxifloxacin (LKT laboratories #M5794) and
20 μg/ml amikacin for 1 hour at room temperature,
incubated at 65 °C for 1 hour, and chilled on ice.
Untreated and antibiotic/heat-treated bacteria were serially diluted and plated
on to LB to determine colony-forming units (CFU). This treatment regimen
resulted in no detectable CFU (limit of detection was 100 CFU/ml).
OD600 = 2. One ml of normalized culture was treated
with 50 μg/ml moxifloxacin (LKT laboratories #M5794) and
20 μg/ml amikacin for 1 hour at room temperature,
incubated at 65 °C for 1 hour, and chilled on ice.
Untreated and antibiotic/heat-treated bacteria were serially diluted and plated
on to LB to determine colony-forming units (CFU). This treatment regimen
resulted in no detectable CFU (limit of detection was 100 CFU/ml).
4
Antimicrobial Agents Evaluation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
DC-159a and sitafloxacin were kindly provided by Daiichi-Sankyo Co., Ltd. (Tokyo, Japan). Moxifloxacin was purchased from LKT Laboratories, Inc. (St. Paul, MN). Ampicillin was purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan).
5
Ocular Antibacterial, Antifungal, and Antiamoebic Agents
6
Cloning and Purification of Mycobacterial Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
FQs, levofloxacin (LVX) and moxifloxacin (MXF) were purchased from LKT Laboratories, Inc. (St Paul, MN, USA) and WQ-3810 was a gift from Wakunaga Pharmaceutical Co., Ltd. (Tokyo, Japan) (Fig. 1). Ampicillin, kanamycin, hygromycin and isoniazid (INH) were purchased from FUJIFILM Wako Pure Chemical Co., Ltd. (Osaka, Japan), ethambutol (EMB) was purchased from MP Biomedicals, LLC (Solon, OH, USA). DNA Ligation Kit, Mighty Mix, and In-Fusion ® HD Cloning Kit were obtained from Takara Bio Inc. (Shiga, Japan). Ni-nitrilotriacetic acid (Ni-NTA) Agarose was obtained from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Restriction enzymes were purchased from New England Biolabs, Inc. (Ipswich, MA, USA). Relaxed pBR322 DNA was purchased from John Innes Enterprises Ltd (Norwich, UK). BD Difco™ Middlebrook 7H9 broth, BD Difco™ Middlebrook 7H10 agar and BD BBL™ Middlebrook OADC Enrichment were obtained from Becton, Dickinson and Co. (MD, USA), and MycoBroth was purchased from Kyokuto Pharmaceutical Industrial Co., Ltd. (Tokyo, Japan). Oligonucleotide primers used for cloning, mutagenesis and sequencing are listed in Supplementary Table S1.
7
Comparative Antimicrobial Efficacy Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
WQ-3810 was kindly provided by Wakunaga pharmaceutical Co., Ltd. (Osaka, Japan).
Moxifloxacin and ofloxacin were purchased from LKT Laboratories, Inc. (St. Paul, MN) and FUJIFILM Wako Pure Chemical Industries Corp. (Osaka, Japan), respectively.
Moxifloxacin and ofloxacin were purchased from LKT Laboratories, Inc. (St. Paul, MN) and FUJIFILM Wako Pure Chemical Industries Corp. (Osaka, Japan), respectively.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!