Log In Sign up
The largest database of trusted experimental protocols

Purified mouse igg

Manufactured by Merck Group
Visit Supplier
Sourced in United States

Purified mouse IgG is a laboratory reagent consisting of immunoglobulin G (IgG) antibodies isolated from mouse serum. It is commonly used as a control or reference material in immunoassays and other applications that require purified mouse antibodies.

Automatically generated - may contain errors

Lab products found in correlation

Anti erk, by Cell Signaling Technology (1 mentions) Cyclic rgdfv, by Enzo Life Sciences (1 mentions) Anti integrin β3, by Cell Signaling Technology (1 mentions) Goat anti rabbit igg h l hrp conjugate secondary antibody, by Bio-Rad (1 mentions) Facs caliber flow cytometer, by BD (1 mentions) Cd8 fitc, by Beckman Coulter (1 mentions)

Close spelling variants of this product

Duolink® In Situ PLA® Probe Anti-Mouse PLUS Affinity purified Donkey anti-Mouse IgG (H+L Rabbit or mouse purified IgG Affinity-purified horseradish peroxidase (HRP)-conjugated anti-mouse IgG IgG mouse

4 protocols using purified mouse igg

1

Integrin-Mediated Cell Signaling Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Chinese hamster ovary (CHO), and MBA-MB-231 human breast cancer cells were obtained from American Type Culture Collection (ATCC). CHO cells expressing human integrin β1 (β1-CHO) or human integrin β3 (β3-CHO) were described [17 (link)]. CHO cells expressing human integrin α6β4 (α6β4-CHO) were described [7 (link)]. Anti-phospho-IGF1Rβ (Tyr-1135 and Tyr1136), anti-IGF1Rβ, anti-phospho-AKT (Ser-473), anti-AKT, anti-phospho-ERK (Thr-202 and Tyr-204), anti-ERK, actin and anti-integrin β3 antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA). The Goat anti-rabbit IgG (H+L)-HRP conjugate (secondary antibody) was purchased from BioRad. HRP-conjugated anti-His tag (C-terminal) antibody was purchased from Qiagen (Valencia, CA). Cyclic RGDfV [18 (link)] was purchased from Enzo Life Sciences (Plymouth Meeting, PA). Poly (2-hydroxyethyl methacrylate) (Poly-HEMA) was purchased from Santa Cruz Biotechnology. The antibody 7E3 (anti-human integrin β3) was obtained from ATCC. Purified mouse IgG was purchased from Sigma. Recombinant soluble integrin ανβ3 was described [19 (link)]. PEI is linear polyethylenimine (MW 25,000) from Polyscience, Inc.
Cedano Prieto D.M., Cheng Y., Chang C.C., Yu J., Takada Y.K, & Takada Y. (2017). Direct integrin binding to insulin-like growth factor-2 through the C-domain is required for insulin-like growth factor receptor type 1 (IGF1R) signaling. PLoS ONE, 12(9), e0184285.
+ Open protocol
+ Expand
2

Cetuximab-IR700 Labeling Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cetuximab-IR700 (cet-IR700) was obtained from the Imaging Probe Development Center (Rockville, MD, USA). IRDye 800CW NHS ester (IR800; C50H54N3Na3O17S4, molecular weight of 1166.2030) was purchased from LI-COR Biosciences (Lincoln, NE, USA). Purified mouse IgG was purchased from SIGMA (Saint Louis, MO, USA). All other chemicals were of reagent grade.
Nagaya T., Sato K., Harada T., Nakamura Y., Choyke P.L, & Kobayashi H. (2015). Near Infrared Photoimmunotherapy Targeting EGFR Positive Triple Negative Breast Cancer: Optimizing the Conjugate-Light Regimen. PLoS ONE, 10(8), e0136829.
+ Open protocol
+ Expand
3

Peripheral Blood Mononuclear Cell Immunophenotyping

Check if the same lab product or an alternative is used in the 5 most similar protocols
Peripheral blood mononuclear cells were resuspended in 0.5% Human Serum Albumin in PBS and 200 ng/ml purified mouse IgG (Sigma) prior to incubation with CD8-FITC (Beckman-Coulter) and either phycoerythrin E75, E90, or negative tetramer (iTAg™ MHC Class I Human Tetramer-SA-PE, (MBL International Corporation, Woburn MA). Stained cells were washed and fixed in 1% formalin (Polysciences) in PBS an hour before acquisition on FACSCaliber™ flow cytometer (BD Biosciences).
, & Stern M.E. (1999). Separation of a midlevel density current from the bottom of a continental slope. Proceedings of the National Academy of Sciences of the United States of America, 96(4), 1206-1211.
+ Open protocol
+ Expand
4

Analyzing FAK/Src/Paxillin Complex Formation

Check if the same lab product or an alternative is used in the 5 most similar protocols
The formation of the FAK/Src/Paxillin complex in MDA-MB-231 cells was analyzed by immunoprecipitation and western blot. Cells were lysed with lysis buffer (1% Triton X-100 in 50 mM Tris-HCl [pH 7.4] containing 150 mM NaCl, 5 mM EDTA, 2 mM Na3VO4, 2.5 mM Na4PO7, 100 mMNaF, 200 nM microcystin lysine-arginine, and protease inhibitors). Cell lysates (300 μg) were mixed with 10 μg of moused anti-FAK monoclonal antibody. Purified mouse IgG (Sigma) was used as the negative control. The samples were incubated for 4 h, mixed with Protein A/G PLUS-agarose immunoprecipitation reagent. (Pierce, Rockford, IL) and then incubated for an additional 12 h. The beads were washed four times, and the bound proteins were released from the beads by boiling in SDS-PAGE sample buffer for 5 min. Samples were analyzed by western blot (described aboved) with rabbit anti-Src polyclonal antibody and rabbit anti-Paxillin polyclonal antibodies.
Yu S., Yang X., Zhu Y., Xie F., Lu Y., Yu T., Yan C., Shao J., Gao Y., Mo F., Cai G., Sinko P.J, & Jia L. (2015). Systems pharmacology of mifepristone (RU486) reveals its 47 hub targets and network: Comprehensive analysis and pharmacological focus on FAK-Src-Paxillin complex. Scientific Reports, 5, 7830.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!