Talos f200s tem

Purified phage particles with a titer of 10¹¹ PFU/mL were loaded onto a copper grid for 1 min, followed by negative staining with uranyl acetate (2% w/v) for 1 min and subsequent aspiration of excess liquid. After drying, the Talos™ F200S TEM (Thermo Fisher Scientific, Waltham, MA, USA) apparatus was used to observe the morphology of phages and to capture images.

The TEM measurements of AuNCs were performed by using a Talos F200S TEM (Thermo Fisher, USA) with an accelerating voltage of 200 kV. The TEM images of the brain slices were performed by using a FEI Tecnai G20 TEM (FEI, USA) with an accelerating voltage of 200 kV. Blinded observation of samples with random selection of grid areas was implemented to reduce bias during imaging.

The morphologies of the samples were observed by a Talos F200S TEM (ThermoFisher, Czech Rep). The hydrodynamic diameters and zeta potentials were measured on a Zetasizer Nano ZS 90 unit (Malvern, UK). X‐ray photoelectron spectroscopy (XPS) was performed on a K‐Alpha photoelectron spectrometer (ThermoScientific, UK). FT‐IR spectra were obtained from a Nicolet iS50 FT‐IR spectrometer (ThermoFisher, UK). ¹H NMR spectra were recorded on an AVANCE NEO 400 spectrometer (Bruker, Switzerland, DMSO‐d6 as solvent). UV–Vis absorption spectra were measured using a UV‐6100 ultraviolet spectrometer (Metash, China). FL spectra were acquired from a RF‐6000 fluorospectrophotometer (Shimadzu, Japan). The purity of the material was determined by a HPLC‐MS/MS method (Agilent, UK). In vitro and in vivo PA images and data were collected by a VEVO LAZR‐X small animal photoacoustic imaging system (Fujifilm VisualSonics, UK). In vitro and in vivo FL images and data were obtained by an IVIS Lumina III small animal optical imaging system (PerkinElmer, UK). MTS results were acquired from a microplate reader (BioTek, UK). Cell uptake was observed by a CytoFLEX LX flow cytometry (Beckman Coulter, UK). Cell FL images were captured on a TCS SP8 DIVE CLSM (Leica, Germany). Cell staining images were obtained from a IX71 inverted fluorescence microscope (Olympus, Japan).