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Ivacaftor vx 770

Manufactured by Selleck Chemicals
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Sourced in Germany, United States

Ivacaftor (VX-770) is a laboratory compound that functions as a potentiator of the cystic fibrosis transmembrane conductance regulator (CFTR) protein. It is used in research and development settings to study the regulation and function of CFTR, which is important in cystic fibrosis and other genetic disorders.

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Lab products found in correlation

Tezacaftor vx 661, by Selleck Chemicals (2 mentions) Cftrinh 172, by Bio-Techne (1 mentions) Caccinh a01, by Bio-Techne (1 mentions) Verapamil, by Merck Group (1 mentions) Iberiotoxin, by Bio-Techne (1 mentions) Gallein, by Bio-Techne (1 mentions) Charybdotoxin, by Bio-Techne (1 mentions) U73122, by Bio-Techne (1 mentions) Mrs 1845, by Bio-Techne (1 mentions) L cis diltiazem, by Enzo Life Sciences (1 mentions) Sq22536, by Abcam (1 mentions)

Spelling variants of this product

VX-770 (67 citations) Ivacaftor (27 citations)

5 protocols using ivacaftor vx 770

1

Formulation and Characterization of PLGA Nanoparticles

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Ciprofloxacin HCl (99.5% purity) was purchased from GenHunter Corporation (Nashville, TN). Ivacaftor (VX-770) was obtained from Selleckchem (Houston, TX). Poly (D, L-lactide-co-glycolide) (PLGA) was purchased from PolySciTech (West Layfeyette, IN). All other chemicals and reagents used in this study were purchased from Sigma-Aldrich (St. Louis, MO)
Cho D.Y., Lim D.J., Mackey C., Weeks C.G., Garcia J.A., Skinner D., Zhang S., McCormick J, & Woodworth B.A. (2019). In Vitro Evaluation of a Ciprofloxacin and Ivacaftor Coated Sinus Stent Against Pseudomonas Aeruginosa Biofilms. International forum of allergy & rhinology, 9(5), 486-492.
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2

Cystic Fibrosis Modulator Screening

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Culture media and supplements used in this article were those described in Gianotti and co-workers [27 (link)]. Ivacaftor (VX770), tezacaftor (VX-661) and elexacaftor (VX-445) were purchased from Selleck Chemicals (Munich, Germany). If not explicitly indicated in the text all other chemicals were provided by Merck (Milan, Italy). CFTR modulators were dissolved in DMSO to have a final concentration of the vehicle in the solutions in contact with the cells ≤ 0.1%.
Ludovico A., Moran O, & Baroni D. (2022). Modulator Combination Improves In Vitro the Microrheological Properties of the Airway Surface Liquid of Cystic Fibrosis Airway Epithelia. International Journal of Molecular Sciences, 23(19), 11396.
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3

Dual-Drug Eluting Biodegradable Stent

Cited 3 times
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Ciprofloxacin HCl (99.5% purity) was purchased from GenHunter Corporation (Nashville, TN). Ivacaftor (VX-770) was obtained from Selleckchem (Houston, TX). Poly (D, L-lactide-co-glycolide) (PLGA) was purchased from PolySciTech (West Layfeyette, IN). All other chemicals and reagents used in this study were procured from Sigma-Aldrich (St. Louis, MO). As previously described, biodegradable poly-D/L-lactic acid (PLLA) tubes were obtained from Zeus Inc (Orangeburg, SC) and laser fine cutting (Laserage Technology Corporation, Waukegan, IL) was employed to create porous stents for the experiments, conforming to the size of the rabbit maxillary sinus, 1 cm in length and 0.3 cm in height.6 (link),11 (link) Eudragit RS 100 (a copolymer of ethyl acrylate, methyl methacrylate and a low content of methacrylic acid ester with quaternary ammonium groups) was used for time-controlled drug release by sustained release formulations.12 (link) Ciprofloxacin (hydrophilic) and ivacaftor (hydrophobic) PLGA nanoparticles were coated in the inner and outer layer respectively.11 (link),13 (link) A total of 0.6mg of ciprofloxacin (10 times higher than the in vitro stent with the AUC0–24/MIC far in excess of the minimum goal (>100)) and 1 mg of ivacaftor (3 times higher than the in vitro stent) were successfully coated on the PLLA stents for the in vivo assay.10 (link)
Lim D.J., McCormick J., Skinner D., Zhang S., Elder J.B., Mclemore J.G., Allen M., West J.M., Grayson J.W., Rowe S.M., Woodworth B.A, & Cho D.Y. (2019). CONTROLLED DELIVERY OF CIPROFLOXACIN AND IVACAFTOR VIA SINUS STENT IN A PRECLINICAL MODEL OF PSEUDOMONAS SINUSITIS. International forum of allergy & rhinology, 10(4), 481-488.
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4

Macrophage Culture and CFTR Modulator Assay

Cited 2 times
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Primary mouse macrophages (BMDMs) were cultured in Iscove’s (IMDM media + 10% fetal bovine serum (FBS)). Cells were treated with CFTR modulators for 24 h at the following concentrations: 10 µM of tezacaftor (VX-661) (S7059, Selleckchem, Houston, TX, USA) and 5 µM of ivacaftor (VX-770) (S1144, Selleckchem). In vitro infections were performed as previously described (Krause et al., 2019 (link); Estfanous et al., 2021 (link)).
Badr A., Eltobgy M., Krause K., Hamilton K., Estfanous S., Daily K.P., Abu Khweek A., Hegazi A., Anne M.N., Carafice C., Robledo-Avila F., Saqr Y., Zhang X., Bonfield T.L., Gavrilin M.A., Partida-Sanchez S., Seveau S., Cormet-Boyaka E, & Amer A.O. (2022). CFTR Modulators Restore Acidification of Autophago-Lysosomes and Bacterial Clearance in Cystic Fibrosis Macrophages. Frontiers in Cellular and Infection Microbiology, 12, 819554.
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5

Comprehensive Odorant Preparation and Pharmacological Inhibition

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Unless otherwise stated, all odorants of the highest purity available were purchased from Sigma-Aldrich (SI Appendix, Table S1). Odorants were freshly prepared on each day of the experiments; they were first reconstituted in DMSO and serially diluted in the appropriate buffer or media. The following pharmacological inhibitors were reconstituted in DMSO, kept at −20 °C, and diluted in the appropriate buffer or media prior to experiments: gallein, U73122, iberiotoxin, charybdotoxin, MRS1845, CaCCinh-A01, H89, CFTRinh-172 (all from Tocris), SQ22536 (Abcam), l-cis diltiazem (Enzo Life Sciences), verapamil (Sigma), and ivacaftor (VX-770, Selleck).
Huang J., Lam H., Koziol-White C., Limjunyawong N., Kim D., Kim N., Karmacharya N., Rajkumar P., Firer D., Dalesio N.M., Jude J., Kurten R.C., Pluznick J.L., Deshpande D.A., Penn R.B., Liggett S.B., Panettieri RA J.r., Dong X, & An S.S. (2020). The odorant receptor OR2W3 on airway smooth muscle evokes bronchodilation via a cooperative chemosensory tradeoff between TMEM16A and CFTR. Proceedings of the National Academy of Sciences of the United States of America, 117(45), 28485-28495.
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