8 br camp

N-[2-[[3-(4-bromophenyl)-2-propenyl]amino]ethyl]-5-isoquinolinesulfonamide dihydrochloride (H89) and 8-Br-cAMP were from R&D Systems (Minneapolis, MN). 2′,5′-dideoxyadenosine (DDA), N,N-dimethyl-(3R,4aR,5S,6aS,10S,10aR,10bS)-5-(acetyloxy)-3-ethenyldodecahydro-10,10b-dihydroxy-3,4a,7,7,10a-pentamethyl-1-oxo-1H-naphtho[2,1-b]pyran-6-yl ester β-alanine hydrochloride (NKH477) and 9-(tetrahydro-2-furanyl)-9H-purin-6-amine (SQ22536) were from Merck Biosciences (Middlesex, UK). [2,8-³H]-adenine was from Perkin Elmer (Waltham, MA). 1,2-bis(o-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (BAPTA) was from Molekula (Gillingham, UK). Carbamylcholine chloride (carbachol, CCh) and 3-isobutyl-1-methylxanthine (IBMX) were from Sigma-Aldrich (Gillingham, UK). Ionomycin was from Apollo Scientific (Stockport, UK). Cell culture media, G-418, fluo 4AM and fura 2AM were from Life Technologies (Paisley, UK). 3-[5-(tert-butyl)isoxazol-3-yl]-2-[2-(3-chlorophenyl)hydrazono]-3-oxopropanenitril (ESI-09) was from Biolog Life Science Institute (Bremen, Germany). All PTH analogues were human forms and supplied by either Bachem (Bubendorf, Switzerland) or, for PTHrP(1-36), custom-synthesized by Selleckchem (Boston, MA, USA). Sequences of the analogues used are listed in supplementary material Table S1.

H89, NKH 477, 8-Br-cAMP, and 8-Br-cGMP were from R&D Systems (Abingdon, Oxford, UK). Sp-cAMPS, 6-Bnz-cAMP, 8-pCPT-2′-O-Me-cAMP, Rp-cAMPS, Rp-8-CPT-cAMPS, ESI-09, and HJC0197 were from Biolog (Bremen, Germany). Ionomycin, SQ 22536, DDA and myristoylated-PKA inhibitor peptide (PKI) were from Merck-Millipore (Watford, UK). A membrane-permeant peptide inhibitor of A kinase-anchoring proteins (AKAPs) [stearated Ht31 AKAP inhibitor peptide (st-Ht31)] and its proline-modified inactive form (st-Ht31P) were from Promega (Southampton, UK). Thapsigargin was from Alomone Laboratories (Jerusalem, Israel). PAR1 peptide, histamine dihydrochloride, forskolin, IBMX, and PGE₂ were from Sigma-Aldrich (Welwyn Garden City, UK). Butaprost (free acid) and L902,688 were from Cayman Chemicals (Ann Arbor, MI). Membrane-permeant caged IP₃ (ci-IP₃PM) was from SiChem (Bremen, Germany). [2,8-³H] adenine ci-IP₃PM, d-2,3-O-isopropylidene-6-O-(2-nitro-4,5-dimethoxy)benzyl-myo-inositol 1,4,5-trisphosphate-hexakis(propionoxymethyl) ester was from Perkin Elmer (Seer Green, Bucks, UK). Fluo-8 was from Stratech Scientific Ltd (Newmarket, Suffolk, UK). Other reagents were from Sigma-Aldrich, sources specified previously (Pantazaka et al., 2013 (link)) or identified in this section.