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Ysc 1 yeast

Manufactured by Merck Group

The YSC-1 yeast is a laboratory equipment product designed for the cultivation and analysis of yeast. It is a compact, self-contained unit that provides a controlled environment for the growth and study of yeast cultures. The YSC-1 supports the fundamental functions of yeast cultivation, including temperature regulation, aeration, and nutrient supply.

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4 protocols using ysc 1 yeast

1

Preparation of Ketogenic Diet for Flies

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Solid CMYD contains 30 g Difco granulated agar (Becton-Dickinson, Sparks, MD), 44 g YSC-1 yeast (Sigma, St. Louis, MO), 328 g cornmeal (Lab Scientific, Highlands, NJ), 400 ml unsulfured Grandma’s molasses (Lab Scientific), 3.6 L water, 40 ml propionic acid (Sigma), and tegosept (8 g Methyl 4-hydroxybenzoate in 75 ml of 95% ethanol) (Sigma). YD contains YSC-1 yeast (Sigma) in water. KD is commercial mouse Teklad ketogenic diet (TD.96355) (Envigo) that contains 173.3 g/Kg casein, 2.6 g/Kg DL-methionine, 586.4 g/Kg vegetable shortening (Crisco), 86.2 g/Kg corn oil, 88.0 g/Kg cellulose, 13.0 g/Kg vitamin mix (Teklad 40060), 2.5 g/Kg choline bitartrate, 0.1 g/Kg tertiary butylhydroquinone (TBHQ), 20.0 g/Kg mineral mix (calcium phosphate deficient), 19.3 g/Kg dibasic calcium phosphate, 8.2 g/Kg calcium carbonate, and 0.4 g/Kg magnesium oxide. KD at 0.3 cal/200 μl was prepared by adding 1.1 g of Teklad ketogenic diet to 5 ml of water and stirring the solution for 1 min at about 95°C. Table 1 provides the caloric contribution of carbohydrate, protein, and fat for each diet as well as the amount of each diet used to make 0.3 cal/200 μl solutions. Flies were fed water and diluted diets by placing 200 μl on a filter paper disc at the bottom of a vial.
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2

Drosophila Maintenance and Fly Line Handling

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All flies were maintained on standard molasses food containing 30 g Difco granulated agar (Becton-Dickinson, Sparks, MD), 44 g YSC-1 yeast (Sigma, St. Louis, MO), 328 g cornmeal (Lab Scientific, Highlands, NJ), 400 ml unsulphured Grandma’s molasses (Lab Scientific), 3.6 L water, 40 ml propionic acid (Sigma), and tegosept (8 g Methyl 4-hydroxybenzoate in 75 ml 95% ethanol) (Sigma). DGRP strains were obtained from the Bloomington Stock Center. The ND2360114 line is an EMS mutant line from the Ganetzky lab temperature-sensitive paralytic collection. Mix sex fly samples contained males and females in a 1:1 ratio (Supplemental Fig. 5). w1118 and DGRP flies were maintained at 25 °C. In Fig. 6, Canton S and ND2360114 flies were maintained at 25 °C and then switched to 29 °C at 0–2 days post-eclosion for the remainder of the experiment. In Supplemental Figs 1 and 6, Canton S flies were maintained at 25 °C.
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3

DGRP Fly Maintenance Protocol

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Drosophila Genetic Reference Panel (DGRP) flies were obtained from the Bloomington Stock Center. The 30 RAL lines that were examined were randomly chosen from the DGRP collection (Mackay et al. 2012 (link)). Flies were maintained on molasses food at 25° unless otherwise stated. Molasses food contained 30 g Difco granulated agar (Becton-Dickinson, Sparks, MD), 44 g YSC-1 yeast (Sigma, St. Louis, MO), 328 g cornmeal (Lab Scientific, Highlands, NJ), 400 ml unsulfured Grandma’s molasses (Lab Scientific), 3.6 liter water, 40 ml propionic acid (Sigma), and tegosept (8 g methyl 4-hydroxybenzoate in 75 ml of 95% ethanol; Sigma). Water vials were prepared immediately before use by placing a circular piece of Whatman filter paper (GE Healthcare Bio-Sciences, Pittsburgh, PA) at the bottom of the vial to absorb 200 μl of water.
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4

Drosophila Maintenance and Antimicrobial Diet

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Flies were maintained on molasses food at 25°C unless otherwise stated. Molasses food contained 30 g Difco granulated agar (Becton-Dickinson, Sparks, MD), 44 g YSC-1 yeast (Sigma, St. Louis, MO), 328 g cornmeal (Lab Scientific, Highlands, NJ), 400 ml unsulphured Grandma's molasses (Lab Scientific), 3.6 l water, 40 ml propionic acid (Sigma), and tegosept (8 g Methyl 4-hydroxybenzoate in 75 ml of 95% ethanol) (Sigma). Water and sucrose, glucose, and fructose (all from Sigma) vials were prepared immediately before use by placing a circular piece of Whatman filter paper (GE Healthcare Bio-Sciences, Pittsburgh, PA) at the bottom of the vial to absorb 200 μl of liquid. Molasses food with antibiotics contained 100 μg/ml ampicillin (Fisher Scientific, Fair Lawn, NJ), 50 μg/ml vancomycin (Sigma), 100 μg/ml neomycin (Sigma), and 100 μg/ml metronidazole (Sigma) in standard molasses food, as described by Liu et al. (2012) (link). The DGRP collection of flies was obtained from the Bloomington Stock Center, the African collection was provided by John Pool (UW-Madison), and grh mutants were provided by Melissa Harrison (UW-Madison) (Mackay et al., 2012 (link); Bastide et al., 2014 (link)).
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