Sw480 cells

Manufactured by Thermo Fisher Scientific

Sourced in United States

The SW480 cells are a well-established human colorectal adenocarcinoma cell line. These cells are commonly used in biological research for various applications.

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SW480 (335 citations) SW480 cell line (2 citations)

9 protocols using sw480 cells

Culturing SW480 and 293T Cell Lines

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The SW480 and 293T cells were purchased from the American Type Culture Collection (Manassas, VA, United States). SW480 cells were maintained in RPMI (Gibco, Life Technologies) supplemented with 10% fetal bovine serum (FBS) (Gibco, Life Technologies) while 293T cells were maintained in DMEM, with 10% FBS. Both cell lines were maintained at 37°C in a humidified incubator containing 5% CO₂.

Abdul S.N., Ab Mutalib N.S., Sean K.S., Syafruddin S.E., Ishak M., Sagap I., Mazlan L., Rose I.M., Abu N., Mokhtar N.M, & Jamal R. (2017). Molecular Characterization of Somatic Alterations in Dukes’ B and C Colorectal Cancers by Targeted Sequencing. Frontiers in Pharmacology, 8, 465.

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Culturing SW480 Colon Cancer Cells

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SW480 cells were obtained from Guangzhou Medical University, which in turn sourced the cell line from the American Type Culture Collection (Manassas, Virginia, USA). SW480 cells were maintained under standard conditions (37°C in 5% atmospheric CO₂) and cultured in DMEM medium (Gibco, Grand Island, New York, USA) containing 10% fetal bovine serum (Gibco). Cell passage was performed every 2–3 days.

Ma W., Liu X, & Du W. (2019). Baicalin induces apoptosis in SW480 cells through downregulation of the SP1 transcription factor. Anti-Cancer Drugs, 30(2), 153-158.

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Cell Culture Maintenance and Density Protocols

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HT29 and HCT116 cells obtained from American Type Culture Collection (ATCC; Manassas, VA, USA) were grown in monolayer cultures and maintained in: McCoys medium (GIBCO/Life Technologies) supplemented with 5% Fetal Bovine Serum (FBS) and 100 units/ml penicillin and 100 ug/ml streptomycin (P/S). SW480 cells obtained from ATCC were maintained in Leibovitz’s 15 (L-15) medium (GIBCO/Life Technologies) supplemented with 5% FBS and P/S. LoVo, Colo320 and DiFi cells were maintained in Roswell Park Memorial Institute (RPMI-1640) medium (GIBCO/Life Technologies) supplemented with 5% FBS and P/S. Hepatocellular carcinoma (HepG2) and Human embryonic kidney cells (HEK293) cell lines were maintained in Dulbecco’s Modified Eagle’s medium (DMEM) supplemented with 5% FBS and P/S. All cell cultures were maintained at 37 °C in humidified air at 5% CO₂. To achieve the desired low and high cell densities, cells were plated at 400,000 cells/100 mm culture dish (Greiner, VWR International) for low density and 800,000 cells/100 mm dishes for high density. Cells were allowed to grow for 3–5 days until a confluency of 30–40% was achieved for low density and 70–80% was achieved for high density. Culture medium for all cell lines was changed every 48 hours. Cell cultures never reached full confluency at the time of analysis. All experiments in this study were conducted within ten passages.

Opdenaker L.M., Modarai S.R, & Boman B.M. (2015). The Proportion of ALDEFLUOR-Positive Cancer Stem Cells Changes with Cell Culture Density Due to the Expression of Different ALDH Isoforms. Cancer studies and molecular medicine : open journal, 2(2), 87-95.

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Colorectal Cancer Cell Lines Culture

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The MC38, CT26, SW480, and HCT116 CRC cell lines and 293FT cells were obtained from American Type Culture Collection (Manassas, VA). MC38 and CT26 cells were cultured with RPMI1640 (Gibco, Shanghai, China), SW480 cells were cultured in L-15 medium, HCT116 cells were cultured in McCoy's 5A medium, and 293FT cells were cultured in high-glucose Dulbecco’s modified Eagle medium (Gibco, Shanghai, China). All medium was supplemented with fetal bovine serum (FBS) (10%; Albany, Australia), penicillin (100 U/mL), and streptomycin (100 mg/mL). The incubator maintained an atmosphere of 5% CO₂ at 37ºC for the MC38, CT26, HCT116, and 293FT cell lines. For the SW480 cell line, CO₂-free culture conditions were used as recommended by American Type Culture Collection.

Yan R., Li J., Xiao Z., Fan X., Liu H., Xu Y., Sun R., Liu J., Yao J., An G., Shi Y, & Ge Y. (2022). DCLK1 Suppresses Tumor-Specific Cytotoxic T Lymphocyte Function Through Recruitment of MDSCs via the CXCL1-CXCR2 Axis. Cellular and Molecular Gastroenterology and Hepatology, 15(2), 463-485.

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Dose-Dependent Effects of NC on Colon Cancer Cells

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Human CC SW480 cells were purchased from China Procell Inc. The cell line SW480 is derived from human colon adenocarcinoma in situ. SW480 cells were added to Dulbecco’s Modified Eagle Medium (DMEM) (Gibco, USA) containing 10% fetal bovine serum (FBS) and cultured at 5% CO₂ at 37 °C. NC (purity >98%) was purchased from Xianxin Biochemical Technology (Sichuan, China). In this study, three concentrations with times ratio (0.25, 0.5, 1 µM) were selected from 0.1 to 1 µM; three concentrations with times ratio (2.5, 5, 10 µM) from 1 to 10 µM; three concentrations with times ratio (25, 50, 100 µM) from 10 to 100 µM, and one concentration (200 µM) from 100 to 200 µM. Different concentrations of NC (0, 0.25, 0.5, 1, 2.5, 5, 10, 25, 50, 100, and 200 µM) were added to the cell-containing medium and cultured for 24 hours.

Gong H., Wang L., Zhao J., Wang L., Yu Q, & Wan Y. (2020). Nitidine chloride inhibits the appearance of cancer stem-like properties and regulates potential the mitochondrial membrane alterations of colon cancer cells. Annals of Translational Medicine, 8(9), 591.

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Cell Culture Maintenance and Density Protocols

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Culturing Human Cancer Cell Lines

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Human hepatocellular carcinoma cell line HepG2 and human colon carcinoma cell line SW480 cell were bought from the Chinese Academy of Science Committee Type Culture Collection Cell Bank (Shanghai, China). HepG2 cells were incubated in DMEM medium (Gibco) and SW480 cells in RPMI 1640 medium (Gibco). The medium was supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin-streptomycin (Invitrogen). Cells were grown at 37 °C in a humidified incubator of 5% CO₂-containing atmosphere.

Gong X., Zheng Y., He G., Chen K., Zeng X, & Chen Z. (2019). Multifunctional nanoplatform based on star-shaped copolymer for liver cancer targeting therapy. Drug Delivery, 26(1), 595-603.

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Transfection of SW480 Cells

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SW480 cells (ATCC) was cultured in Dulbecco’s modified Eagle’s Medium (DMEM, Life Technologies) supplemented with 10% fetal bovine serum and penicillin–streptomycin (Life Technologies) at 37 °C incubator with 5% CO₂.
For transfection, cells were plated on 0.1% gelatin (EMD Millipore) coated cover glasses (Warner Instruments, CS-12R 12 mm) in a 24 well cell culture plate (Thermo Fisher). Transfection was done using lipofectamine 3000 reagent (Thermo Fisher) following manufacturer’s manual. Amount of plasmids used for each well of a 24 well plate: mEGFP or mEGFP-APC fragment, 150 ng; mCherry-Axin, 50 ng; FLAG-β-catenin, 150 ng.

Li T.M., Ren J., Husmann D., Coan J.P., Gozani O, & Chua K.F. (2020). Multivalent tumor suppressor adenomatous polyposis coli promotes Axin biomolecular condensate formation and efficient β-catenin degradation. Scientific Reports, 10, 17425.

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Cell Culture and Authentication

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SW480 cells (ECACC, cell line authentication report number 710236782) were cultured in DMEM medium (Life Technologies, #61965) supplemented with 10% fetal calf serum; H460 cells (ECACC, cell line authentication report number 710236782) were grown in RPMI medium (Life Technologies, #61870) supplemented with 10% fetal calf serum. Both cell lines have undergone 16 loci STR authentication (LGC Standards, UK).

Jezkova J., Williams J.S., Jones-Hutchins F., Sammut S.J., Gollins S., Cree I., Coupland S., McFarlane R.J, & Wakeman J.A. (2014). Brachyury regulates proliferation of cancer cells via a p27Kip1-dependent pathway. Oncotarget, 5(11), 3813-3822.

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