Goat polyclonal secondary antibody to rabbit igg h l alexa fluor 647

Quercetin (Cat.no.Q4951-10G) was purchased from Sigma (St.Louis, MO, United States) with >95% purity (HPLC). The protein assay reagent kit bicinchoninic acid (BCA), trypsin-EDTA, fetal bovine serum (FBS) and Dulbecco’s Modified Eagle Medium (DMEM) were obtained from Thermo Fisher Scientific (Waltham, MA United States). Phosphate saline buffer was purchased from Maixin Biotechnology (Fuzhou, Fujian China), and polyvinylidene fluoride (PVDF) membrane was purchased from Millipore (Billireca, MA, United States). Antibodies against STAT3 (Cat no.41464), p-JAK2 (Cat no.11151) were purchased from Signaling antibody (SAB) (College Park, Maryland, United States). The α-SMA (Cat no.19245S), p-STAT3 (Cat no.9145S), JAK2 (Cat no. 3032S) antibodies were purchased from Cell Signaling Technology (Danvers, MA, United States). PCNA (Cat no. ab29), goat polyclonal secondary antibody to rabbit IgG H&L (Alexa Fluor^® 647; Cat no. ab150079), Ang II (Cat no. ab120183) were purchased from Abcam (Cambridge Science Park, Cambridge, United Kingdom) and anti-rabbit secondary antibody (Cat no. 7074) was purchased from Cell Signaling Technology (Danvers, MA, United States). Antibody against GAPDH (Cat no. Abp57259) and kit of CCK-8 (Cat no. ktc011001) were obtained from Abbkine (Wuhan, Hubei, China).

Hydrogen Tetrachloroaurate(III) Trihydrate (HAuCl₄·3H₂O) and methylene blue (MB) were obtained from Shanghai Titan Technology Co., Ltd. Copper(II) sulfate pentahydrate (CuSO₄·5H₂O), selenium dioxide (SeO₂), sodium citrate dihydrate, ascorbic acid, and hydrogen peroxide (H₂O₂) were purchased from Sinopharm Chemical Reagent Co., Ltd. Polyvinylpyrrolidone (PVP, Mw ~55,000), 2′,7′- dichlorofluorescein diacetate (DCFH-DA), and Calcein AM were provided by Sigma Aldrich. Roswell Park Memorial Institute 1640 (RPMI-1640) was supported by Corning Incorporated. Fetal bovine serum (FBS), trypsin, and penicillin-streptomycin solution were supplied from Gibco Company. Cell Counting Kit-8 (CCK-8) solution was purchased from Dojindo Laboratories. Hoechst 33,342, 4′,6-diamidino-2-phenylindole (DAPI), and propidium iodide (PI) were acquired from Beyotime Biotechnology. Mitochondrial hydroxyl radical detection assay, anti-gamma H2AX (phospho S139) antibody [EP854(2)Y], and goat polyclonal secondary antibody to rabbit IgG - H&L (Alexa Fluor® 647) were purchased from Abcam Company. Accutase™ Cell Detachment Solution and FITC Annexin V Apoptosis Detection Kit were supplied from BD Bioscience. All chemicals and reagents were used as received without further purification.