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Pva dabco mounting solution

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PVA-DABCO mounting solution is a laboratory product designed for use in microscopy and imaging applications. It is a water-soluble, non-hardening mounting medium that helps preserve and stabilize samples for observation. The solution contains polyvinyl alcohol (PVA) and 1,4-diazabicyclo[2.2.2]octane (DABCO) as key components.

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DABCO (258 citations) PVA-DABCO (24 citations) Mounting solution (16 citations) PVA solution (4 citations) DABCO solution (2 citations)

2 protocols using pva dabco mounting solution

1

Immunofluorescence Staining Protocol

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Cells were washed in PBS, fixed with 4% paraformaldehyde (PFA) for 20 min at room temperature and blocked with 5% horse serum in PBS containing 0.1% TritonX-100. Primary antibodies were incubated overnight at 4ºC, washed three times with PBS, incubated with secondary antibodies for two hours at room temperature, stained with DAPI solution and mounted in PVA-DABCO mounting solution (Sigma Aldrich). For confocal imaging, cells were grown on ibidi μ-slides. Antibodies and respective concentrations are listed in the Supplementary Methods.
Schafer S.T., Paquola A.C., Stern S., Gosselin D., Ku M., Pena M., Kuret T.J., Liyanage M., Mansour A.A., Jaeger B.N., Marchetto M.C., Glass C.K., Mertens J, & Gage F.H. (2019). Pathological priming causes developmental gene network heterochronicity in autism patient-derived neurons. Nature neuroscience, 22(2), 243-255.
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2

Immunofluorescence Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were washed in PBS, fixed with 4% paraformaldehyde (PFA) for 20 min at room temperature and blocked with 5% horse serum in PBS containing 0.1% TritonX-100. Primary antibodies were incubated overnight at 4ºC, washed three times with PBS, incubated with secondary antibodies for two hours at room temperature, stained with DAPI solution and mounted in PVA-DABCO mounting solution (Sigma Aldrich). For confocal imaging, cells were grown on ibidi μ-slides. Antibodies and respective concentrations are listed in the Supplementary Methods.
Schafer S.T., Paquola A.C., Stern S., Gosselin D., Ku M., Pena M., Kuret T.J., Liyanage M., Mansour A.A., Jaeger B.N., Marchetto M.C., Glass C.K., Mertens J, & Gage F.H. (2019). Pathological priming causes developmental gene network heterochronicity in autism patient-derived neurons. Nature neuroscience, 22(2), 243-255.
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