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2 protocols using albumin bovine serum bsa

1

Investigating Cellular Signaling Pathways

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The MEK inhibitor PD184352, the AKT inhibitor AKTi, Epidermal Growth Factor (EGF), basic Fibroblast Growth Factor (bFGF), Polyethylenimine (PEI), Avidin-FITC and 4′6-diamino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich (Rehovot, Israel). Protein A/G PLUS-agarose beads were obtained from Santa Cruz Biotechnology, Inc. (CA, USA). Glutathione beads were purchased from GE-healthcare and Proximity ligation assay kit was from Olink Bioscience (both Uppsala, Sweden). Albumin bovine serum (BSA) was purchased from MP biomedical (OH, USA). Si RNAs and Dharmafect were from Thermo Fisher Scientific (CO, USA). NBT/BCIP developing substrates were purchased from Promega (Wis, USA). Soybean trypsin inhibitor (SBTI) was purchased from Biological Industries (Beit HaEmek, Israel).
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2

Molecular Signaling Pathway Analysis

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Tetradecanoyl phorbol acetate (TPA), poly-L-Lysine (PLL) and 4,6-diamino-2-phenylindole (DAPI) were purchased from Sigma-Aldrich (Rehovot, Israel). Albumin bovine serum (BSA) was purchased from MP biomedical (OH, USA). The BRAF inhibitor PLX4032 and MEK inhibitor Trametinib (GSK1120212) were purchased from SelleckChem (Huston, TX). CellTiter-Glo reagent was purchased from Promega (Madison, WI).
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