Dmem f12k medium

Noscapine, docetaxel were purchased from Sigma Chemicals, St. Louis, MO, USA and AK Scientific Chemicals, CA, USA. The TNBC cell lines MDA-MB-231 cells were obtained from American Type Culture Collection (Rockville, MD, USA). Cells were grown in DMEM:F12K medium (Sigma, St. Louis, MO, USA) supplemented with 10% fetal bovine serum and antibiotic solution purchased from life technologies, USA. The cells were maintained at 37 °C in the presence of 5% CO₂. All other chemicals were either reagent or tissue culture grade.

BT20, A549, and THP-1 (ATCC, USA) were maintained in RPMI 1640 medium (Sigma-Aldrich, USA). MCF-7 and MDA-MB-231 (ATCC, USA) were maintained in Dulbecco's Modified Eagle Medium (DMEM) with 2 mM L-glutamine (Sigma-Aldrich, USA). HMEC and U2OS (ATCC, USA) were maintained in McCoy's 5A medium (ATCC, USA). MCF-10A (ATCC, USA) was maintained in MEBM basal medium (Lonza corporation, USA) supplemented with the MEGM SingleQuot Kit (Lonza corporation, USA) and 100 ng/ml cholera toxin (Sigma-Aldrich, USA). HPL1A was obtained from Nagoya University, Japan, and maintained in DMEM/F-12K medium (Sigma-Aldrich, USA). HUVEC (ATCC, USA) was maintained in Vascular Cell Basal Medium (ATCC, USA) supplemented with the Endothelial Cell Growth Kit-BBE (ATCC, USA). All mediums were supplemented with 10% fetal bovine serum (FBS) (Life Technologies, USA) and 1% penicillin-streptomycin (Sigma-Aldrich, USA) and all cells were incubated in a humidified atmosphere with 5% CO₂ at 37°C.