Wound tissue samples were excised at the size of 1.0 cm × 1.0 cm on day 2 and day 8 post-wounding and biochemically analyzed to estimate endogenous antioxidant enzyme and lipid peroxidation activities. Each tissue sample was dried and weighed prior to use for analysis. Tissues were transferred to a bead tube with Tris-buffer and homogenized using a microtube homogenizer (Benchmark Scientific, USA). The homogenized tissues were then centrifuged at 6000 rpm for 20 min and we collected the supernatant for the biochemical estimations. Activity levels of endogenous antioxidant enzymes such as superoxide dismutase (SOD) (Item No 706002; Cayman) and glutathione peroxidase (GPx) (Item No 703102; Cayman) and lipid peroxidation, measured as malondialdehyde (MDA) content (Item No 10009055; Cayman), were measured following the vendor’s protocol.
Microtube homogenizer
Manufactured by Benchmark Scientific
Sourced in United States
The Microtube homogenizer is a laboratory instrument designed for the efficient homogenization of small sample volumes, typically in the range of 0.2 to 2 milliliters. The device utilizes a high-speed motor to drive a stainless steel shaft and blade assembly, providing thorough mixing and disruption of samples within microtubes or small vials.
Automatically generated - may contain errors
Lab products found in correlation
Malondialdehyde mda, by Cayman Chemical (1 mentions)
Radio immunoprecipitation assay ripa buffer, by Thermo Fisher Scientific (1 mentions)
Complete mini proteinase inhibitor cocktail tablets, by Roche (1 mentions)
Biorad image lab 4, by Bio-Rad (1 mentions)
Anti mbp, by Fortrea (1 mentions)
Chemidoc xrs imaging system, by Bio-Rad (1 mentions)
2 protocols using microtube homogenizer
1
Assessing Wound Antioxidant and Lipid Peroxidation
2
Western Blot Analysis of Myelin Basic Protein
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