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Ab201510

Manufactured by Abcam

AB201510 is a laboratory equipment product. It is a multi-purpose device used for various applications in scientific research and analysis. The core function of this product is to perform specific tasks related to the handling, processing, or measurement of samples in a laboratory environment.

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2 protocols using ab201510

1

Comprehensive Epigenetic Regulation Analysis

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Western blot and ChIP analyses were performed with: anti-BAP1 (D7W70; Cell Signaling), anti-Suz12 (D39F6; Cell Signaling Technology), anti-FLAG (F3165; Sigma-Aldrich), anti-H3K27me3 (9733; Cell Signaling Technology), anti-H2AK119ub1 (8240; Cell Signaling Technology), anti-H3 (ab1791; Abcam), anti-H2A (07-146; Sigma-Aldrich), anti-RING1B (homemade (Chiacchiera et al., 2016 (link))), anti-PCGF1, anti-PCGF2, and anti-PCGF6 (Pasini’s lab, homemade (Scelfo et al., 2019 (link))), anti-JARID2 (13594, Cell Signaling Technology), anti-AEBP2 (14129; Cell signaling Technology), anti-HA (C29F4, Cell Signaling Technology), anti-ASXL2 (A302-037A, Bethyl), anti-ASXL1 (D1B6V, Cell Signaling Technology) anti-KDM1B/LSD2 (E1R60, Cell Signaling Technology), anti-OGT (SC-32921, Santa-Cruz), anti-HCFC1 (A301-400A, Bethyl), anti-FOXK2 (A301-730A, Bethyl), anti-MBD6 (SAB1305225-40TST, Sigma-Aldrich), anti-H3K27ac (AB4729, Abcam) anti-PCGF3/5 (AB201510, Abcam), anti-UTX (Helin lab, homemade (Agger et al., 2007 (link))), anti-MTF2 (Proteintech 16208-1-AP), anti-CBX7 (abcam, ab21873), anti-RYBP/DEDAF (Sigma-Aldrich, PRS2227), anti-H3K36me2 (abcam, ab9049), anti-RING1B-pS168 (abcam, ab234421).
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2

Proteomic Profiling of PHF10 Knockdown

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This was performed with standard approaches by querying a set of proteins identified following PHF10 knockdown in cell lines. Antibodies were: PHF10 (ab154637 Abcam), JAZF1 (ab80329 Abcam), SMAD2 (ab40855 Abcam) and PCGF3/5 (ab201510 Abcam).
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