Mtorc2

Primary antibodies against Bcl-2, Bax, Bad, and Bcl-xL were procured from Abcam (USA). Akt, p-Akt, mTORC1, p-mTORC1, mTORC2, p-mTORC2, phosphatase and tensin homolog (PTEN), glycogen synthase kinase 3β (GSK-3β), p- GSK-3β (Ser9), NF-κBp65, and cleaved caspase-3 were procured from Cell Signaling Technology (Danvers, MA, USA). CyclinD1, β-actin, and horseradish- and peroxidase-labeled IgG secondary antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA) and used for expression analysis. Cell lysis buffer for Western blotting analysis was purchased from Beyotime Institute of Biotechnology (Beijing, China). Mangiferin and isoflurane were procured from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals used for analysis were obtained from Sigma-Aldrich.

PTZ (P6500) (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in freshly prepared saline solution prior to administration. Antibodies against Akt, p-Akt, GSK-3β, mTORc1, p-mTORc1, mTORc2, p-mTORc2, cleaved caspase-3, BDNF, TrkB and nGAD65 were purchased from Cell Signaling Technology (Danvers, MA, USA). GABA_A, p-GSK-3β, Phosphatase and tensin homolog (PTEN), Bax, Bcl-2, Bad, Bcl-xL and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA) and were used for expression analysis. Nobiletin and the remaining chemicals were obtained from Sigma-Aldrich.