Chemstation lc 3d rev b 04

Agilent Technologies 1200 Series HPLC system equipped with a G1315C DAD, G1316B column compartment, G1367D autosampler, and G1312B binary pump was used to analyse the acidic and neutral forms of phytocannabinoids. The separation was performed on a Phenomenex Kinetex EVO C18 column (5 μm, 100 x 2.1 mm id) with a Phenomenex SecurityGuard ULTRA guard column. Instrument control, data acquisition, and integration were done with ChemStation LC 3D Rev B.04.02 software (Agilent Technologies). A 2 μL injection volume was used for all calibration standards (THC, CBD, THC-A, CBD-A, CBG, CBG-A, all Sigma-Aldrich) and sample analysis. The compound peaks were detected for 230 nm and 280 nm. Mobile phases consisted of 50 mM ammonium formate (pH 5.19) (Sigma-Aldrich) in HPLC grade water (Fisher Chemical) on the A side and 100% methanol (Fisher Chemical) on the B side, with a flow rate 0.3 ml/min. Two samples per cultivar were analyzed, with two technical repeats per each sample. Data are presented in Table 3.

Agilent Technologies 1200 Series HPLC system, equipped with a G1315C DAD, G1316B column compartment, G1367D autosampler, and G1312B binary pump was used to analyze the acidic and neutral forms of phytocannabinoids. The separation was performed on a Phenomenex Kinetex EVO C18 column (5 µm, 100 × 2.1 mm id) with a Phenomenex SecurityGuard ULTRA guard column. Instrument control, data acquisition, and integration were carried out with ChemStation LC 3D Rev B.04.02 software (Agilent Technologies, Santa Clara, United States). A 2 µL injection volume was used for all calibration standards (THC, CBD, THC-A, CBD-A, CBG, CBG-A, all Sigma-Aldrich, Montreal Canada) and sample analysis. The compound peaks were detected at 230 nm and 280 nm. Mobile phases consisted of 50 mM ammonium formate (pH 5.2) (Sigma-Aldrich) in HPLC grade water (ThermoFisher Scientific, Toronto, Canada) on the A side and 100% methanol (Fisher Chemical) on the B side, with a flow rate 0.3 mL/min. Two samples per each flower sample were analyzed, with two technical repeats per each sample.