yohimbine HCl (Tocris) was administered in a dose (1.0mg/kg) able to potentiate fear memory consolidation (Gazarini et al., 2013 (link)). The lipophilic β-adrenoceptor antagonist propranolol HCl (10mg/kg) and the hydrophilic β-adrenoceptor antagonist nadolol (10mg/kg) were purchased from Sigma-Aldrich and used to investigate the role of central and peripheral β-adrenoceptors in the enhanced noradrenergic activity induced by yohimbine. In any case, the dose selected was able to prevent the facilitating effects of other β-adrenoceptor agonists on fear memory consolidation (Gazarini et al., 2013 (link)). Clonidine HCl (Sigma-Aldrich; 0.3mg/kg) and cannabidiol (THC-Pharma; 10mg/kg) were administered at putative memory reconsolidation-disrupting doses (Stern et al., 2012 (link), Gazarini et al., 2013 (link)). D-cycloserine (Sigma-Aldrich) was administered in a dose (15mg/kg) able to potentiate fear memory labilization (Bustos et al., 2010 (link)). All drugs were dissolved in 0.9% NaCl, except for cannabidiol, which was dissolved in NaCl 0.9% containing 5% of Tween 80® (Vetec) and administered systemically in a volume of 1.0ml/kg.
Yohimbine hcl
Manufactured by Bio-Techne
Yohimbine HCl is a pure, crystalline alkaloid extracted from the bark of the Pausinystalia yohimbe tree. It is commonly used as a laboratory reagent and research chemical. Yohimbine HCl has physiological effects and may be used in various research applications.
Automatically generated - may contain errors
Lab products found in correlation
D cycloserine, by Merck Group (1 mentions)
Yohimbine, by Merck Group (1 mentions)
Nadolol, by Merck Group (1 mentions)
Clonidine hcl, by Merck Group (1 mentions)
Propranolol hcl, by Merck Group (1 mentions)
Tween 80, by Vetec (1 mentions)
Naloxone hcl, by Merck Group (1 mentions)
Rimonabant sr141716a, by Bio-Techne (1 mentions)
2 protocols using yohimbine hcl
1
Adrenergic Modulation of Fear Memory
2
Pharmacological Modulation of Nociception
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Naloxone HCl (Sigma) was prepared as a stock (50 mg/ml) in distilled water and diluted in 0.9% saline vehicle before systemic (1 mg/kg, i.p.) [26, 53] or spinal (10 µg, i.t.) [69] administration. Yohimbine HCl (Tocris) was prepared as a stock (10 mg/ml) in DMSO and diluted in 0.9 saline (30% DMSO vehicle) for spinal (30 µg, i.t.) [39] administration. S-sulpiride HCl (Sigma) was prepared as a stock (30 mg/ml) in DMSO and diluted in saline (10% DMSO vehicle) for systemic (10 mg/kg, i.p.) [9] administration. The CB1 receptor antagonist, rimonabant (SR141716A) (Tocris) was prepared as a stock in DMSO and diluted in a vehicle containing 2% tween-80 and 20% DMSO in 0.9% saline 45] for systemic administration (5 mg/kg, i.p.) [37, 73] . Sensory testing was performed 10-15 mins after systemic and spinal injections and completed within 45 min of drug treatment. The same animals underwent alternative treatment of drug or vehicle solutions separated by at least one rest day. Spinal drug treatments were followed by a 20 µl saline flush. All testing was performed blind to the treatment.
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