Chemagic msm 1 system

Manufactured by PerkinElmer

Sourced in Canada

The Chemagic MSM I system is an automated magnetic separation system designed for high-throughput nucleic acid extraction and purification from various sample types. The system utilizes magnetic bead-based technology to streamline the sample preparation process, providing consistent and reliable results.

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Lab products found in correlation

2019 ncov cdc ddpcr triplex probe assay, by Bio-Rad (2 mentions) One step rt ddpcr advanced kit for probe, by Bio-Rad (2 mentions) Autodg system, by Bio-Rad (2 mentions) Quantasoft analysis pro v1, by Bio-Rad (2 mentions) C1000 thermocycler, by Bio-Rad (2 mentions) Qx200 droplet reader, by Bio-Rad (2 mentions) Biomek nxp, by Beckman Coulter (1 mentions) Epitect 96 bisulfite kit, by Qiagen (1 mentions) Trusight cancer sequencing panel, by Illumina (1 mentions) Miseq platform, by Illumina (1 mentions) 7900ht fast real time pcr system, by Thermo Fisher Scientific (1 mentions)

6 protocols using chemagic msm 1 system

SARS-CoV-2 RNA Extraction and ddPCR Detection

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Viral nucleic acid was extracted from 200 μL of heat-inactivated SARS-CoV-2 stock using the chemagic MSM I system (PerkinElmer, Santa Clara, CA) and eluted in a total volume of 80 μL. ddPCR was performed using the one-step RT-ddPCR advanced kit for probes (Bio-Rad, Hercules, CA) with the 2019-nCoV CDC ddPCR triplex probe assay (catalog number dEXS28563542; Bio-Rad) targeting the N1 and N2 genes, following the manufacturer’s protocol. Briefly, droplet generation was performed with the AutoDG system (Bio-Rad), and amplification was performed with a C1000 thermocycler (Bio-Rad); plates were read with a QX200 droplet reader (Bio-Rad), and results were analyzed manually using QuantaSoft Analysis Pro v1.0.596 (Bio-Rad).

Gavina K., Franco L.C., Robinson C.M., Hymas W., Lei G.S., Sinclair W., Hall T., Carlquist J., Lavik J.P., Emery C.L., Heaton P.R., Hillyard D., Lopransi B.K, & Relich R.F. (2023). Standardization of SARS-CoV-2 Cycle Threshold Values: Multisite Investigation Evaluating Viral Quantitation across Multiple Commercial COVID-19 Detection Platforms. Microbiology Spectrum, 11(1), e04470-22.

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SARS-CoV-2 RNA Extraction and ddPCR Detection

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Interlaboratory DNA Extraction and Analysis

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DNA samples used for the 2016 and 2018 AFCTs were extracted from dog whole blood, as well as cattle and fish meat, using the Chemagic™ MSM I System (Perkin Elmer, Waltham, MA, USA) at the Duty Laboratory (Eurofins Genomics, Ebersberg, Germany). DNA concentrations were determined using a DropSense 96 polychromatic spectrophotometer (Trinean, Pleasanton, CA, USA), and the DNA sample dilutions were performed with a Hamilton Microlab Star Plus Liquid Handling System (Hamilton, Reno, NV, USA). The laboratories participating in the 2016 and 2018 AFCTs were located in Argentina, Czech Republic, France, Germany, Italy, Japan, the Netherlands, Poland, South Africa, Slovenia, Spain, USA and Uruguay. Ten laboratories submitted their results in 2016 and 2018 tests. All AFCT results and survey data were submitted by these laboratories directly to the Computer Laboratory (Identitas Laboratory, Montevideo, Uruguay) for analysis.

Kanthaswamy S., Brendel T., Cancela L., Andrade de Oliveira D.A., Brenig B., Cons C., Crespi J.A., Dajbychová M., Feldl A., Itoh T., Landi V., Martinez A., Natonek-Wisniewska M., Oldt R.F., Radko A., Ramírez O., Rodellar C., Ruiz-Girón M., Schikorski D., Turba M.E, & Giovambatista G. (2021). An inter-laboratory study of DNA-based identity, parentage and species testing in animal forensic genetics. Forensic Sciences Research, 7(4), 708-713.

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DNA Methylation Analysis from Blood

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DNA for methylation analysis of both cohorts was extracted from blood. The cleanup of genomic DNA from healthy participants was performed using the Nucleo-Mag® Blood 200 μl DNA Kit (Macherey-Nagel, Düren, Germany). Genomic DNA from Crohn patients was extracted according to standard procedures with an automated chemagic MSM I system (Perkin Elmer, Baesweiler, Germany). Bisulfite conversion and DNA purification were conducted via the EpiTect® 96 Bisulfite Kit (QIAGEN, Hilden, Germany). DNA concentrations were determined via a Nanodrop 1000 spectrophotometer (VWR, Radnor, PA, USA). A Biomek® NxP (Beckman Coulter, Brea, CA, USA) was used for pipetting, transferring and purification steps.

Gombert S., Rhein M., Winterpacht A., Münster T., Hillemacher T., Leffler A, & Frieling H. (2019). Transient receptor potential ankyrin 1 promoter methylation and peripheral pain sensitivity in Crohn’s disease. Clinical Epigenetics, 12, 1.

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Genetic Diagnostics for BRCA1/2 Mutations

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DNA from peripheral blood lymphocytes was extracted with an automated chemagic MSM I system according to standard procedures (Perkin Elmer, Baesweiler, Germany). Mutational analysis of BRCA1/2 genes was performed either with Sanger sequencing and MLPA analysis for copy number variant (CNV) identification (MRC-Holland, Amsterdam, Netherlands) or with Next Generation Sequencing on a MiSeq platform (Illumina, San Diego, CA). The commercially available targeted resequencing kit, TruSight Cancer Sequencing Panel (Illumina, San Diego, CA), was used according to the manufacturers’ instructions. Sequencing reads were aligned and processed following standard clinical grade genetic diagnostics as previously described [42 (link)]. The targeted genes had an average coverage of 400 reads. Complete coverage (> 30 reads) was obtained for the coding regions and the 10 bp of flanking intronic regions.

Vasileiou G., Costa M.J., Long C., Wetzler I.R., Hoyer J., Kraus C., Popp B., Emons J., Wunderle M., Wenkel E., Uder M., Beckmann M.W., Jud S.M., Fasching P.A., Cavallaro A., Reis A, & Hammon M. (2020). Breast MRI texture analysis for prediction of BRCA-associated genetic risk. BMC Medical Imaging, 20, 86.

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Genotyping BDNF Val66Met Polymorphism

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Genomic DNA was extracted from ethylenediaminetetraacetic acid anti-coagulated venous blood samples using the chemagic MSM I system (PerkinElmer Chemagen Technologie GmbH). BDNF Val66Met (rs6265) was genotyped on a 7900HT Fast Real-Time PCR System (Life Technologies) using TaqMan®SNP Genotyping Assay C-11592758_10 (Life Technologies) and the standard protocol for allelic discrimination. Accuracy was assessed by duplicating 15% of the original sample, and reproducibility was 100%.

Strube W., Nitsche M.A., Wobrock T., Bunse T., Rein B., Herrmann M., Schmitt A., Nieratschker V., Witt S.H., Rietschel M., Falkai P, & Hasan A. (2015). BDNF-Val66Met-Polymorphism Impact on Cortical Plasticity in Schizophrenia Patients: A Proof-of-Concept Study. International Journal of Neuropsychopharmacology, 18(4), pyu040.

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