Snap kp sil cartridge

Manufactured by Biotage

The SNAP KP-Sil Cartridge is a laboratory equipment designed for solid-phase extraction (SPE) applications. It is a chromatographic column filled with a silica-based sorbent material, which is used to isolate and purify target analytes from complex sample matrices. The SNAP KP-Sil Cartridge provides a reliable and efficient method for sample preparation prior to further analytical procedures.

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Lab products found in correlation

60f254 silica gel plate, by Merck Group (1 mentions) Isolera one automated, by Biotage (1 mentions)

4 protocols using snap kp sil cartridge

Chromatographic Purification of Tetrakis-Substituted Zinc Phthalocyaninate

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Example 7

Starting From a Batch of Compound (II) With the Following Isomers Distribution:

A=6.7%, B=6.5%, C+D=86.8%;

Performing the Chromatography Purification (Step ii)) as Described:

1.8 g of [1,8(11),15(18),22(25)-tetrakis-(3-N,N-dimethylaminophenoxy)] zinc phthalocyaninate (II) are dissolved in 12 ml of an eluent mixture X/Y 3/1 in which X=DCM/THF/MeOH 94/5/1 e Y=n-hexane. The sample is loaded on a chromatographic pre-packed column (Biotage SNAP KP-Sil Cartridge, silica gel 100 g, mesh 40-63 μm) pre-conditioned with X/Y 3/1. The elution (Flow: 40 ml/min) is performed with X/Y 3/1 until ail isomer B is eluted, then ratio of the mixture X/Y is carried to 4/1 and so kept for the elution of isomers C, D and A. The selected fractions are collected and dried to give 1.4 g of [1,8(11),15(18),22(25)-tetrakis-(3-N,N-dimethylaminophenoxy)] zinc phthalocyaninate “with low isomer B content” (III) with the following isomers distribution are obtained: A=7.4%, B=0.4%, C+D=92.2%;

Performing Steps iii) and iv) on This Batch of Compound (III):

a batch of compound (I) with the following isomers distribution is obtained: A=7.6%, B=0.5%, C=62.6%, D=29.3%.

US10730890B2. Phthalocyanine derivative consisting of a mixture of 4 isomers (2020-08-04). MOLTENI THERAPEUTICS S.R.L. [IT]. Inventors: Gabrio Roncucci [IT], Donata Dei [IT], Giacomo Chiti [IT], Daniele Nistri [IT].

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Isolation and Purification of Compound 1 from Aspergillus violaceofuscus

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Aspergillus violaceofuscus CBS 115571 was purchased from the Westerdijk Fungal Biodiversity Institute, inoculated on 100 YES agar plates [ca. 2 L; 20 g/L yeast extract, 150 g/L sucrose, 0.5 g/L MgSO₄⋅7H₂O, 20 g/L agar supplemented with 1mL/L of a trace element solution (10 g/L ZnSO₄⋅7H₂O, 5 g/L CuSO₄⋅5H₂O), pH 6.5], and cultivated for 9 days at 25°C. The resultant fungal cultures including agar were crushed into small pieces and extracted with ethyl acetate twice using an ultrasonic bath. The crude extract was subjected to flash chromatography with Biotage^® SNAP KP-Sil cartridge (100 g) and eluted stepwise using chloroform:ethyl acetate gradient (100:0 to 0:100). Fractions that contained 1 were concentrated and further purified by reverse-phase preparative HPLC (40% aqueous acetonitrile containing 0.05% trifluoroacetic acid, 10 mL/min) to yield 64.9 mg of colorless crystals.

Wei X., Chen L., Tang J.W, & Matsuda Y. (2020). Discovery of Pyranoviolin A and Its Biosynthetic Gene Cluster in Aspergillus violaceofuscus. Frontiers in Microbiology, 11, 562063.

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Synthesis of Clickable Lipid Conjugates

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Unless stated otherwise, all reagents and
chemicals were obtained from commercial sources at the highest purity
available and used without further purification. All solvents were
of AR quality and purchased from Biosolve. Water was purified on an
EMD Millipore Milli-Q Integral Water Purification System. Reactions
were followed by thin-layer chromatography (precoated 0.25 mm, 60-F254
silica gel plates from Merck). Dry solvents were obtained with an
MBRAUN Solvent Purification System (MB-SPS). Ion exchange resin DOWEX
1X8-50 (Cl-form) was obtained from Acros. Prior to use, a column was
first washed with demineralized water, followed by washing with methanol.
Weakly basic resin Amberlite IRA-95 (Aldrich) was washed with water,
methanol, and again water before use. Automated column chromatography
was performed on a Biotage Isolera using Biotage SNAP-KP SIL cartridges.
H₂N-C₁₂-EO₄-N₃,^{18 (link)} H₂N-C₁₂-EO₄-OBn,^{13 (link)} Chol-NHS,^{35 (link)} Atr-C₅-OH*HCl,^{36 (link)} DMT-MM,^{37 (link)} 5-methoxycarbonyl-benzene-1,3-dicarboxylic acid
(3),^{38 (link)} and BTA-(OH)₃(13 (link)) were synthesized according
to previously published procedures.

Vleugels M.E., Varela-Aramburu S., de Waal B.F., Schoenmakers S.M., Maestro B., Palmans A.R., Sanz J.M, & Meijer E.W. (2021). Choline-Functionalized Supramolecular Copolymers: Toward Antimicrobial Activity against Streptococcus pneumoniae. Biomacromolecules, 22(12), 5363-5373.

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Optimized Procedures for Organic Synthesis

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All reactions were performed in single-neck, oven-dried (130 °C for 12 h), round-bottomed flasks fitted with rubber septa under a positive pressure of argon, unless otherwise specified. Organic solutions were concentrated by rotary evaporator at 30–50 °C, as noted. Analytical thin-layered chromatography (TLC) was performed using plates pre-coated with silica gel (Sigma Aldrich; 60 Å, 17 μm particle size), impregnated with a fluorescent indicator (254 nm). TLC plates were visualized by exposure to ultraviolet (UV) light, submersion in butanolic ninhydrin solution, or basic potassium permanganate solution, followed by brief heating with a Master-Mite heat gun (hot setting, 30 s). Flash-column chromatography was performed using a Biotage Isolera One automated flash purification system with Biotage SNAP KP-Sil cartridges.

Nikolayevskiy H., Robello M., Scerba M.T., Pasternak E.H., Saha M., Hartman T.L., Buchholz C.A., Buckheit RW J.r., Durell S.R, & Appella D.H. (2019). The structure-activity profile of mercaptobenzamides’ anti-HIV activity suggests that thermodynamics of metabolism is more important than binding affinity to the target. European journal of medicinal chemistry, 178, 818-837.

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