Alexa fluor 647 conjugated goat anti human igg h l antibody

PbPf sporozoites were treated as described for the in vitro cytotoxicity assay in suspension with 100 mg/mL of each monoclonal antibody and 10% FCS/PBS. After 45 min at 37°C, samples were placed on ice, transferred to an 18-well slide (iBidi), fixed with 2% Formaldehyde (Thermo Fisher Scientific) for 20 min at room temperature, blocked and incubated with 4 mg/mL Alexa Fluor ^™ 647-conjugated goat anti-human IgG (H + L) antibody (Invitrogen) in PBS with 1% BSA for 40 min at room temperature. After washing, wells were filled with mounting medium (iBidi). Images were acquired with an inverted Axio Observer Z.1 microscope (Zeiss) and analyzed using Fiji.^{37 (link)}