Honey was purchased from Patanjali. Ghee was purchased from Anik. SGOT, SGPT, ALP, urea, Creatinine, BUN, glucose estimation kits were purchased from Accurex Biomedical PVT LTD., Biosar, Thane. Lipase substrate, 4-nitrophenyl butyrate, DPP-4 substrate gly-pro-p-nitroanilide, and ABTS+ tablet were purchased from Sigma-Aldrich. GLP-1 and GIP EIA kit were purchased from Sigma-Aldrich. Nitroblue tetrazolium chloride, metaphosphoric acid (HPO3)n, Methionine, Riboflavin, Bradford reagent, thiobarbituric acid, Bromocresol green (BCG) reagent, Trichloro-acetic acid were purchased from Hi-media Ltd, Calcutta, Hemocord-D, a hemoglobin reagent was purchased from Coral Clinical System, Goa.
Riboflavin
Manufactured by HiMedia
Sourced in India
Riboflavin, also known as vitamin B2, is a water-soluble vitamin that plays a crucial role in various metabolic processes within the human body. It serves as a cofactor for enzymes involved in energy production, antioxidant function, and the metabolism of fats, proteins, and carbohydrates.
Automatically generated - may contain errors
Lab products found in correlation
Methionine, by HiMedia (3 mentions)
Thiobarbituric acid, by HiMedia (2 mentions)
Ferric chloride, by HiMedia (2 mentions)
Ethylenediaminetetraacetic acid, by HiMedia (2 mentions)
Nitro blue tetrazolium, by HiMedia (2 mentions)
4 nitrophenyl butyrate, by Merck Group (1 mentions)
Abts tablet, by Merck Group (1 mentions)
Nitroblue tetrazolium chloride, by HiMedia (1 mentions)
Bradford reagent, by HiMedia (1 mentions)
Trichloroacetic acid, by HiMedia (1 mentions)
Ascorbic acid, by HiMedia (1 mentions)
Gallic acid, by HiMedia (1 mentions)
Cisplatin, by Thermo Fisher Scientific (1 mentions)
Sulphanilamide, by HiMedia (1 mentions)
Hydrogen peroxide, by Thermo Fisher Scientific (1 mentions)
Uv vis spectrophotometer, by Shimadzu (1 mentions)
L tyrosine, by HiMedia (1 mentions)
Pectin, by HiMedia (1 mentions)
Ferrous sulphate, by HiMedia (1 mentions)
Kinetin, by HiMedia (1 mentions)
6 protocols using riboflavin
1
Biochemical Characterization of Honey and Ghee
2
Antioxidant Capacity Evaluation Protocol
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1,1-Diphenyl,2-picryl hydrazyl (DPPH), nitroblue tetrazolium (NBT), riboflavin, L-methionine, thiobarbituric acid (TBA), ethylenediaminetetraacetic acid (EDTA), ascorbic acid, gallic acid, rutin and trichloroacetic acid (TCA), potassium ferricyanide (“K3[Fe(CN)6]”), and ferric chloride (“FeCl3”) were purchased from Hi-Media Ltd. All reagents were of analytical grade.
3
Cisplatin-Induced Oxidative Stress Markers
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Cisplatin was purchased from Thermo Fisher, Waltham, USA. Sulphanilamide, EDTA (Ethylenediamine- tetra acetic acid disodium salt dihydrate), NBT (Nitroblue tetrazolium chloride), Methionine, Riboflavin were procured from Himedia, Mumbai, India. Creatinine assay kit (Erba) was purchased from Transasia Bio-medicals Ltd., Solan, H.P. India, and Urea assay Kit (Auto span) was purchased from Arkray healthcare Pvt. Ltd., Gujrat, India, Hydrogen peroxide was obtained from Fisher Scientific Mumbai, India. All other chemicals were of analytical grade and were purchased from local vendors supplying scientific grade chemicals.
4
Mushroom-Derived Enzymatic Characterization
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Mushrooms (Agaricus bisporus) were procured from the local supermarket. L-Tyrosine and riboflavin were purchased from HiMedia Chemicals, Mumbai, India. Ammonium sulfate, calcium phosphate gel, p-cresol, catechol, Folin's reagent, glutaraldehyde, bovine serum albumin, N, N′ methylene bisacrylamide, acrylamide monomer, and sodium alginate were purchased from SRL India Pvt. Ltd. All other chemicals used in this investigation were of analytical grade. All the experiments were carried out in triplicates. The absorbance of samples was recorded in a Shimadzu UV-Vis spectrophotometer.
5
Phytochemical Analysis and Antioxidant Evaluation
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Murashige and Skoog (MS) medium, kinetin, naphthalene acetic acid, pectin, MnCl2, methanol, toluene, chloroform, ethanol, diethyl ether, benzene, streptomycin, ciprofloxacin, doxycycline, ampicillin, ofloxacin, dimethyl sulfoxide, DPPH (1,1-diphenyl-2-picrylhydrazyl), sodium acetate, glacial acetic acid, hydrochloric acid, TPTZ (2,4,6-tri[2-pyridyl]-s-triazine), ferrous sulphate, ferric chloride, nitro blue tetrazolium, ethylenediaminetetraacetic acid (EDTA), riboflavin, hydrogen peroxide, methionine, dipotassium phosphate, monopotassium phosphate, and thymol (THY) were purchased from HIMEDIA; thymoquinone (TQ) was purchased from Sigma Aldrich.
6
Microcrystalline Cellulose and Azo Dye Synthesis
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Microcrystalline cellulose and sulfanilic acid (≥99.0%) were purchased from respectively, Brazil) . Riboflavin (Fig. 1a), succinic anhydride, acetic anhydride, acetic acid, pyridine, sodium hydroxide, ethanol, acetone, and methylene chloride were purchased from Vetec (Brazil). Hydrochloric acid, diethyl ether, N,Ndimethylformamide (DMF), and dimethylsulfoxide (DMSO) were purchased from Synth (Brazil). Remazol Golden Yellow RNL (RGY-RNL, C 16 H 18 N 4 O 10 S 3 Na 2 , Fig. 1a) azo dye was kindly provided by a local textile industry and used without further purification. Yeast extract containing 50 g/g of Riboflavin was purchased from Himedia.
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