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Env 230

Manufactured by Med Associates

The ENV-230 is a lab equipment product designed for environmental monitoring and analysis. It serves as a versatile tool for researchers and technicians working in areas such as air quality, water treatment, and soil testing. The core function of the ENV-230 is to provide accurate and reliable data collection for environmental assessment and monitoring purposes.

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2 protocols using env 230

1

Operant Conditioning Chamber Protocol

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Experimental sessions were conducted in two standard operant conditioning chambers controlled by Med-PC IV software (Med Associates; St. Albans, VT) as described in detail previously (Bertz and Woods, 2013 (link)). Each chamber was contained inside a light- and sound-attenuating cubicle and was located in a separate room of the laboratory. The right wall of each chamber contained a white incandescent houselight (ENV-215M, Med Associates) and the speaker for a tone generator (ENV-224AM and ENV-230, Med Associates). Two nose-poke manipulanda containing LED stimulus lights (ENV-114BM, Med Associates) could also be inserted into the right wall. When the nose-pokes were removed from the chamber, they were replaced by blank aluminum panels.
Motorized syringe drivers (PHM-107, Med Associates) were located outside of the light- and sound-attenuating cubicles to deliver IV drug injections. Syringes were attached to Tygon tubing (S-54-HL, Norton Performance Plastics) leading to a counterweighted fluid swivel (375/22PS, Instech Laboratories) and spring tether.
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2

Operant Conditioning Chamber Setup for Instrumental Training

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Instrumental training and testing took place in four identical operant conditioning chambers (29.5 × 32.5 × 24.5 cm; Med Associates, East Fairfield, Georgia, VT) located in the colony room. The chamber was placed within sound- and light-attenuating housing equipped with a ventilation fan that also screened external noise. Inside the chamber, two (left and right) retractable levers (4.7 cm wide) were mounted on a panel 11 cm apart and 6 cm from the grid floor. A pellet dispenser (ENV-007CT, MED Associates) delivered individual 45 mg food pellets (Bio-Serve, Frenchtown, NJ) into a recessed magazine situated between the levers. The dispenser could be controlled manually by pressing a button on the outside of the cage. The chamber was equipped with a tone generator (ENV-230, MED Associates) and a speaker that was positioned 18 cm above the grid floor, on the wall opposite the levers. A white house light (2.5 W, 24 V) was also located on this wall. A charge coupled device camera was placed on the ceiling of the chambers to monitor the behavior of the animals. Control of the chambers and collection of the data were accomplished by MED-PC IV software (Med Associates) on a personal computer running the Windows XP operating system, which was connected to the operant chambers via a set of four interfaces.
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