Citrate buffer

Manufactured by Biocare Medical

Citrate buffer is a commonly used solution in laboratory settings. It serves as a buffer to maintain a specific pH range, typically between 3.0 and 6.2. The buffer composition helps stabilize the pH of the solution, which is essential for various biochemical and analytical procedures.

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Lab products found in correlation

Tcs sp5 spectral confocal microscope, by Leica (1 mentions) Rabbit anti gr antibody, by Cell Signaling Technology (1 mentions) Alexa fluor 488 goat anti mouse igg, by Thermo Fisher Scientific (1 mentions) Eclipse te300, by Nikon (1 mentions) Background sniper solution, by Biocare Medical (1 mentions) Decloaking chamber, by Biocare Medical (1 mentions) Mayer s hematoxylin, by Merck Group (1 mentions)

Spelling variants of this product

Citrate-buffer-based antigen retrieval medium (2 citations) Citrate Buffer pH 6.0 (2 citations)

2 protocols using citrate buffer

Cardiac Histology and Immunofluorescence

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Hearts from control and cardioGRKO mice were perfused with PBS and fixed with 4% paraformaldehyde. Samples were processed, embedded in paraffin, cut in 5 μm sections, and stained with hematoxylin and eosin. Pictures were acquired using a Nikon Eclipse TE300 inverted microscope. For the immunofluorescence, Citrate buffer (Biocare Medical, CA) was used for antigen retrieval and blocked with 5% normal goat serum in 0.2% triton/PBS. After blocking, samples were incubated overnight with rabbit anti‐GR antibody (Cell Signaling, MA) and mouse anti‐troponin I antibody (Millipore, MA) followed by 2‐hour incubation with a goat anti‐rabbit IgG antibody, Alexa Fluor 594 (red) and a goat anti‐mouse IgG, Alexa Fluor 488 (green) from ThermoFisher Scientific. Nuclei was stained with DAPI. Images were obtained on a Leica TCS SP5 Spectral Confocal Microscope equipped with a ×40 (oil) objective.

Cruz‐Topete D., Oakley R.H., Carroll N.G., He B., Myers P.H., Xu X., Watts M.N., Trosclair K., Glasscock E., Dominic P, & Cidlowski J.A. (2019). Deletion of the Cardiomyocyte Glucocorticoid Receptor Leads to Sexually Dimorphic Changes in Cardiac Gene Expression and Progression to Heart Failure. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 8(15), e011012.

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Immunohistochemistry and Immunofluorescence Staining Protocol

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Immunohistochemical and immunofluorescence stainings were performed on 4μm-thick FFPE tissue sections. Sections were treated with xylene and graded alcohols and antigen retrieval was performed in citrate buffer (Biocare Medical) using a Decloaking Chamber (Biocare Medical). Regular immunohistochemical staining was performed using Cell and Tissue Mouse HRP kit (R&D) and counterstained with Mayer’s hematoxylin (Sigma-Aldrich). For immunofluorescence, background staining was blocked with Background Sniper solution (Biocare Medical) after antigen retrieval. The following antibodies were used for immunohistochemistry and immunofluorescence: anti-human CD20 (L26, Dako), CD20 (BV11, Abcam), Cytokeratin (AE1AE3, Abcam), CD31 (EPR3094, Abcam), CD34 (EP373Y, Abcam), CD138 (SP152, Abcam), Secretory IgG (EPR4421, Abcam). Primary antibodies were diluted in TBS with 1% BSA and incubated overnight at 4°C. For immunofluorescence, control stainings with fluorochrome-conjugated secondary antibodies only are presented in Fig. S15.

Nuñez S., Moore C., Gao B., Rogers K., Hidalgo Y., del Nido P.J., Restaino S., Naka Y., Bhagat G., Madsen J.C., Bono M.R, & Zorn E. (2016). The human thymus perivascular space is a functional niche for viral-specific plasma cells. Science immunology, 1(6), eaah4447.

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