Isodca

Manufactured by Merck Group

IsoDCA is a laboratory equipment product designed for analytical applications. It is a high-performance liquid chromatography (HPLC) column used for the separation and analysis of various chemical compounds. The core function of IsoDCA is to facilitate the precise and efficient separation of complex mixtures, enabling researchers and analysts to accurately identify and quantify the components within a sample.

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Lab products found in correlation

Allolca, by Merck Group (2 mentions) 3 oxodca, by Merck Group (2 mentions) Qual browser, by Thermo Fisher Scientific (1 mentions) 3 oxolca, by Steraloids (1 mentions)

2 protocols using isodca

LC-MS Identification of Bile Acid Derivatives

Cited 2 times

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The raw LC-MS data were acquired using the same C18-negative mode LC-MS methods described in the HMP2 and PRISM studies^{26 (link),27 (link),46 (link)}. Peaks of unknown ID were confirmed using authentic standards run alongside with the quality control reference stool pool generated in the HMP2 study. The LCA derivatives were confirmed by matching their m/z in negative mode and retention time, and subsequently verified using LC-MS/MS (Fig. S8). Extracted ion chromatograms (EICs) were generated using QualBrowser (Xcalibur 4.1.31.9; Thermo Fisher Scientific, Waltham, MA). The commercial standards used are: LCA (Sigma, L6250), 3-oxoLCA (Steraloids (C1750–000, isoLCA (Steraloids, C1475–000), isoalloLCA (Steraloids, C0700–000), alloLCA (Steraloids, C0680–000), DCA (Sigma, D2510), 3-oxoDCA (Steraloids, C1725–000), and isoDCA (Steraloids, C1165–000). LCA peak in PRISM: FFA_Cluster_0731, m/z = 375.2898 at 12.42 min, and in HMP2: C18n_QI48, m/z = 375.2905 at 11.98 min; 3-oxoLCA in PRISM: FFA_Cluster_0722, m/z = 373.2744 at 12.63 min and in HMP2: C18n_QI6169, m/z = 373.2749 at 12.2–12.35 min; IsoLCA in PRISM: FFA_Cluster_0733, m/z = 375.2901 at 11.73 min and in HMP2: C18n_QI6230, m/z = 375.2906 at 11.31 min (Fig. S8, Table S12).

Paik D., Yao L., Zhang Y., Bae S., D’Agostino G.D., Zhang M., Kim E., Franzosa E.A., Avila-Pacheco J., Bisanz J.E., Rakowski C.K., Vlamakis H., Xavier R.J., Turnbaugh P.J., Longman R.S., Krout M.R., Clish C.B., Rastinejad F., Huttenhower C., Huh J.R, & Devlin A.S. (2022). Human gut bacteria produce TH17-modulating bile acid metabolites. Nature, 603(7903), 907-912.

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Bile Acid Profiling by LC-MS

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The raw LC-MS data were acquired using the same C18-negative mode LC-MS methods described in the HMP2 and PRISM studies 29, 44 . Peaks of unknown ID were confirmed using authentic standards run alongside with the quality control reference stool pool generated in the HMP2 study. The LCA derivatives were confirmed by matching their m/z in negative mode and retention time, and subsequently verified using LC-MS/MS (Fig. S8). Extracted ion chromatograms (EICs) were generated using QualBrowser (Xcalibur 4.1.31.9; Thermo Fisher Scientific, Waltham, MA). The commercial standards used are: LCA (Sigma, L6250), 3-oxoLCA (Steraloids (C1750-000, isoLCA (Steraloids, C1475-000), isoalloLCA (Steraloids, C0700-000), alloLCA (Steraloids, C0680-000), DCA (Sigma, D2510), 3-oxoDCA (Steraloids, C1725-000), and isoDCA (Steraloids, C1165-000). LCA peak in PRISM: FFA_Cluster_0731, m/z = 375.2898

Paik D., Yao L., Zhang Y., Bae S., D’Agostino G.D., Kim E., Franzosa E.A., Ávila-Pacheco J., Bisanz J.E., Rakowski C.K., Vlamakis H., Xavier R.J., Turnbaugh P.J., Longman R., Krout M.R., Clish C.B., Huttenhower C., Huh J.R., & Devlin A.S. (2021). Human gut bacteria produce T_H17-modulating bile acid metabolites.

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