Urinary and plasma creatinine measurement is routinely and stably performed with an IDMS traceable enzymatic assay (Multigent; Abbott) since February 2011. From 2005 to 2011, urinary and plasma creatinine was measured with a colorimetric assay. Contrary to plasma creatinine, previous studies have demonstrated an excellent agreement between colorimetric and enzymatic assays for the measurement of urinary creatinine in adult patients presumably because of the lack of interfering chromogens in urine.28 CER was determined as the mean of the values obtained on successive urine samples collected every hour for 5 h. Total calcium, phosphate, urea, standard plasma, and urine ion and protein concentration were measured by standard biochemical methods. Ionized calcium was measured on an ABL800 analyser (Radiometer). C‐terminal fibroblast growth factor 23 (FGF23) plasma concentration was measured by ELISA (Immutopics). Serum parathyroid hormone concentration was measured with an immunochemiluminescent assay performed on the Elecsys analyser (RocheDiagnostics). Calcitriol and 25‐hydroxy vitamin D (25‐OH‐vitD) concentrations were measured with radioimmunoassays (DiaSorin). Osteocalcin and beta‐cross‐laps were measured with Cobas® immunoassays (Roche). The assays were carried out in a single laboratory throughout the study.
Elecsys analyser
Manufactured by Roche
Sourced in Germany
The Elecsys analyser is a fully automated immunoassay system designed for the in vitro quantitative determination of various analytes in biological samples. It utilizes electrochemiluminescence (ECL) technology to perform immunoassay tests. The core function of the Elecsys analyser is to provide accurate and reliable results for clinical diagnostic applications.
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Lab products found in correlation
2 protocols using elecsys analyser
1
Detailed Biomarker Measurement Protocol
2
Comprehensive Evaluation of Sickle Cell Disease
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All screening study subjects were evaluated by histories of clinical events and lifetime treatments, physical examination, laboratory screening, transthoracic Doppler echocardiography, and the six-minute walk test. Routine laboratory tests (complete blood count, serum chemistry profile, and lactate dehydrogenase) from samples taken at the subject's screening visit were performed in the local laboratories of the participating institutions. Echocardiography was performed at the participating institutions and read centrally in the NHLBI echocardiography core laboratory. Percentage of hemoglobin F was measured by high-performance liquid chromatography (HPLC) (Ultra Resolution System, Trinity Biotech). Alpha-thalassemia was detected by molecular methodology based on polymerase chain reaction at the University of Pittsburgh. Serum N-terminal pro-brain natriuretic peptide (NT-pro BNP) concentration was measured by a sandwich immunoassay using polyclonal antibodies that recognize epitopes located in the N-terminal segment (1–76) of pro-BNP (1–108) (Elecsys analyser; Roche Diagnostics, Mannheim, Germany), as previously described [13] . Ferritin was measured with an enzyme immunoassay (Ramco Laboratories Inc, Stafford, TX; reference range, 20–300 ng/mL).
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