Mice were anesthetized with 0.1 ml/10 g of body weight dose of 2.0% tribromoethanol (Sigma Aldrich, St. Louis, MO, USA) and transcardially perfused with PBS followed by 4% paraformaldehyde. Tissues were postfixed in 4% PFA overnight at 4°C, cryoprotected by submersion in 30% sucrose in PBS for 16–24 h, then embedded in OCT, and cryosectioned for immunofluorescence, and hematoxylin (Fisher Chemical, SH26-500D) and eosin (Sigma-Aldrich, HT110132-1L) staining was performed. Pathological and histological analyses for Atxn10Cagg pancreata, kidneys, spleen, retina, lungs, and liver were performed by the Comparative Pathology Lab (UAB) as follows. Briefly, mice were necropsied, and tissues were fixed in 10% neutral-buffered formalin overnight. Tissues were prosected and processed, then 5 µM sections were stained with hematoxylin and eosin. Slides were evaluated for tissue histopathology by a board-certified veterinary pathologist in blinded fashion.
Ht110132 1l
Manufactured by Merck Group
HT110132-1L is a laboratory product from Merck Group. It is a liquid substance packaged in a 1-liter container. No further details about its core function or intended use can be provided while maintaining an unbiased and factual approach.
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3 protocols using ht110132 1l
1
Comprehensive Histopathological Analysis of Atxn10 Mouse Model
2
Histological Examination of Spleens and Kidneys
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Spleens and kidneys were fixed in 10% (v/v) normal buffered formalin, then dehydrated via a Tissue Processor (Leica, TP1020) and embedded in paraffin (Leica, EG1150H). Two micrometer sections were cut (Microm HM335E) and stained with a hematoxylin (Mayer's hematoxylin; Applichem, APC2-254766.1611) and eosin (Eosin Y solution; Sigma, HT110132-1L) stain. Kidneys were also stained with periodic acid (3%, Morphisto, 11839.00500) and Schiff's reagent (Carl Roth, X900.1). Images were taken with Axio Imager A1 from Zeiss.
3
Comprehensive Histopathological Analysis of Atxn10 Cagg Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were anesthetized with 0.1 ml/ 10 g of body weight dose of 2.0% tribromoethanol (Sigma Aldrich, St. Louis, MO) and transcardially perfused with PBS followed by 4% paraformaldehyde.
Tissues were post-fixed in 4% PFA overnight at 4°C, cryoprotected by submersion in 30% sucrose in PBS for 16-24 hours, then embedded in OCT, and cryosectioned for immunofluorescence, and Hematoxylin (Fisher Chemical, SH26-500D) and Eosin (Sigma-Aldrich, HT110132-1L) staining was performed. Pathological and histological analyses for Atxn10 Cagg pancreata, kidneys, spleen, retina, lung, and liver were performed by the Comparative Pathology Lab (UAB) as follows.
Briefly, mice were necropsied and tissues were fixed in 10% neutral buffered formalin overnight.
Tissues were prosected and processed then 5 µM sections were stained with Hematoxylin and Eosin. Slides were evaluated for tissue histopathology by a board certified veterinary pathologist in blinded fashion.
Tissues were post-fixed in 4% PFA overnight at 4°C, cryoprotected by submersion in 30% sucrose in PBS for 16-24 hours, then embedded in OCT, and cryosectioned for immunofluorescence, and Hematoxylin (Fisher Chemical, SH26-500D) and Eosin (Sigma-Aldrich, HT110132-1L) staining was performed. Pathological and histological analyses for Atxn10 Cagg pancreata, kidneys, spleen, retina, lung, and liver were performed by the Comparative Pathology Lab (UAB) as follows.
Briefly, mice were necropsied and tissues were fixed in 10% neutral buffered formalin overnight.
Tissues were prosected and processed then 5 µM sections were stained with Hematoxylin and Eosin. Slides were evaluated for tissue histopathology by a board certified veterinary pathologist in blinded fashion.
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