Recombinant human coronavirus 229E spike protein (Sinobiological, China) was used to immunize two female 16-week-old New Zealand rabbits (Pizhou Dongfang, China) three times with 2-week intervals. Whole blood was subjected to antibody affinity purification. Caco2 and Rhileki cells were seeded at a concentration of 2 × 106 cells before infection. Polyclonal antisera were serially diluted 2-fold and were incubated with 126 TCID50 of C2, C2R10, and C2R10C1 viruses for 1 h at 33°C. Subsequently, Caco2 and Rhileki cells were inoculated for 2 h at 33°C, medium was replenished, and virus titers were quantified at 3 dpi.
Recombinant human coronavirus 229e spike protein
Manufactured by Sino Biological
Sourced in China
Recombinant human coronavirus 229E spike protein is a laboratory reagent produced by Sino Biological. The spike protein is a structural protein found on the surface of the human coronavirus 229E, which is a common cause of the common cold. This recombinant protein can be used for research purposes, such as the development of diagnostic tests or the study of virus-host interactions.
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Lab products found in correlation
2 protocols using recombinant human coronavirus 229e spike protein
1
Rabbit-derived Antibodies Neutralize SARS-CoV-2 Variants
2
Rabbit-derived Antibodies Neutralize SARS-CoV-2 Variants
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Recombinant human coronavirus 229E spike protein (Sinobiological, China) was used to immunize two female 16-week-old New Zealand rabbits (Pizhou Dongfang, China) three times with 2-week intervals. Whole blood was subjected to antibody affinity purification. Caco2 and Rhileki cells were seeded at a concentration of 2 × 106 cells before infection. Polyclonal antisera were serially diluted 2-fold and were incubated with 126 TCID50 of C2, C2R10, and C2R10C1 viruses for 1 h at 33°C. Subsequently, Caco2 and Rhileki cells were inoculated for 2 h at 33°C, medium was replenished, and virus titers were quantified at 3 dpi.
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