Quantikine scd14 immunoassay

As is described elsewhere (28 (link), 34 (link)), blood samples were collected at one time point between 2005–2006 using serum separator and EDTA tubes and were shipped to a central repository at the Massachusetts Veterans Epidemiology Research and Information Center. Assays for biomarkers examined here were conducted at the Laboratory for Clinical Biochemistry Research at the University of Vermont and used four controls per sample to assess interassay coefficients of variability (CVs). sCD14 was quantified by an enzyme-linked immunosorbent assay (Quantikine sCD14 Immunoassay, R&D Systems, Minneapolis, MN) with a detectable range of 40–3,200 ng/ml. The interassay CVs ranged from 7.2–8.1%. IL-6 was quantified by a chemiluminescent immunoassay (QuantiGlo IL-6 immunoassay, R&D Systems, Minneapolis, MN) with detectable range of 0.4–10,000 pg/mL. The interassay CVs ranged from 7.7–12.3%. D-dimer was quantified by a STAR automated coagulation analyzer (Diagnostica Stago, Parsippany, NJ) using an immunoturbidometric assay (Liatest D-DI) with a detectable range of 0.01–20 ug/mL. The interassay CVs ranged from 2.8–14.8%. All three biomarkers were natural log transformed before analysis to approximate a normal distribution.