Mg132 a2585

Manufactured by Apexbio

Sourced in United States

MG132 (A2585) is a laboratory reagent used in biochemical research. It functions as a proteasome inhibitor, preventing the degradation of proteins by the proteasome complex. The core function of MG132 is to disrupt the normal protein degradation processes within cells, which can be useful for studying cellular pathways and protein dynamics.

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Lab products found in correlation

Polyclonal rabbit anti hif 1α, by Abcam (2 mentions) Polyclonal rabbit anti vhl, by Proteintech (2 mentions) Mouse monoclonal anti ha, by Cell Signaling Technology (2 mentions) Monoclonal mouse anti flag, by Abcam (2 mentions) Chx a8244, by Apexbio (2 mentions) Bay85 3934, by Apexbio (2 mentions) Mouse monoclonal anti gapdh, by Abcam (2 mentions) Rabbit polyclonal anti caspase 3, by Cell Signaling Technology (2 mentions) Polyclonal goat anti septin4, by Abcam (2 mentions) Anti ha antibody h3663, by Merck Group (1 mentions) Anti β actin a1978, by Merck Group (1 mentions) Anti ha tag sc805, by Santa Cruz Biotechnology (1 mentions) Leptomycin b l2913, by Merck Group (1 mentions) Cycloheximide c1988, by Merck Group (1 mentions) Rhodamine phalloidin phdr1, by Cytoskeleton (1 mentions) Anti flag m2 f1804, by Merck Group (1 mentions)

5 protocols using mg132 a2585

Quantifying Protein Interactions via Immunoblotting

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The following antibodies were used: anti‐caspase3 (D3R6Y, mAb #14220, Cell Signaling Technology, WB: 1:1000), anti‐Smurf2 (D8B8, mAb #12024, Cell Signaling Technology, WB: 1:1000, IF: 1:50), anti‐PARP1 (46D11, mAb #9532, Cell Signaling Technology, WB: 1:1000, IF: 1:200), anti‐MY (9B11, mAb #2276, Cell Signaling Technology, WB: 1:1000), anti‐Flag(9A3, mAb #8146, Cell Signaling Technology, WB: 1:1000, IF: 1:50), anti‐HA (6E2, mAb #2367, Cell Signaling Technology, WB: 1:1000) and anti‐β‐tubulin (10094‐1‐AP, Proteintech, WB: 1:1000). Protein A/G magnetic beads were acquired from Biotool. Cycloheximide (A8244) and MG132 (A2585) were purchased from ApexBio. Heclin (5433) was purchased from Tocris a bio‐techne brand.

Qian H., Zhang N., Wu B., Wu S., You S., Zhang Y, & Sun Y. (2020). The E3 ubiquitin ligase Smurf2 regulates PARP1 stability to alleviate oxidative stress‐induced injury in human umbilical vein endothelial cells. Journal of Cellular and Molecular Medicine, 24(8), 4600-4611.

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Analyzing STOP1 Protein Levels in Arabidopsis Roots

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Twelve-old-day seedlings of Col-0 WT, rae2, and rae3 harboring pSTOP1:STOP1-HA transgene were pretreated with a 0.5mM CaCl₂ solution at pH 4.8 for 6 h and then treated with the same solution containing 0 or 30 μM Al at pH 4.8 for 12 h. Total proteins were extracted from the roots or shoots of each line using a extraction buffer composed of 20 mM Tris–HCl (pH 7.4), 300 mM NaCl, 5 mM MgCl₂ (pH 8.0), 5 mM DTT, 0.5% NP-40, 50 μM MG132 (A2585; APExBIO, United States), and 1× Complete Protease inhibitor tablets EDTA-free (5892791001; Roche). Immunoblot analysis was performed to compare STOP1-HA protein level using anti-HA antibody (H3663; Sigma-Aldrich).

Zhu Y.F., Guo J., Zhang Y, & Huang C.F. (2021). The THO/TREX Complex Component RAE2/TEX1 Is Involved in the Regulation of Aluminum Resistance and Low Phosphate Response in Arabidopsis. Frontiers in Plant Science, 12, 698443.

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Protein Expression and Analysis

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Polyclonal goat anti-Septin4 (abcam, USA), polyclonal rabbit anti-HIF-1α (abcam), polyclonal rabbit anti-VHL (proteintech, USA), polyclonal rabbit anti-caspase-3 (Cell Signaling Technology, USA), monoclonal mouse anti-HA (Cell Signaling Technology), monoclonal mouse anti-Flag (abcam), monoclonal rabbit anti-β-tubulin (Proteintech), and monoclonal mouse anti-GAPDH (abcam). CHX (A8244) and MG132 (A2585), used in DMSO, were purchased from ApexBio (USA). HIF-PHDs inhibitor BAY85-3934 was also purchased from ApexBio.

Wu S., Zhang Y., You S., Lu S., Zhang N, & Sun Y. (2021). Septin4 promotes cardiomyocytes apoptosis by enhancing the VHL-mediated degradation of HIF-1α. Cell Death Discovery, 7, 172.

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Antibody Characterization and Reagent Validation

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Anti-HDAC1 antibody was previously generated in our laboratory^{69 (link)}. Anti-FLAG M2 (F1804) and anti-β-actin (A1978) antibodies were purchased from Sigma-Aldrich (St. Louis, MO). Anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, HRP-60004) antibody was from Proteintech (Rosemont, IL). Anti-Lamin A (sc-20680) and anti-HA tag (sc-805) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX). Anti-EGFR (A300-388A) antibody was from Bethyl Laboratories (Montgomery, TX). Anti-phospho-EGFR (Y1173) (AF1095) antibody was from R&D Systems (Minneapolis, MN). Anti-Bim (2876) and anti-Myc tag (9B11, 2276) antibodies were from Cell Signaling Technology (Danvers, MA). Phosphotyrosine antibody cocktail (13-6620) was from Invitrogen (Thermo Fisher Scientific, Waltham, MA). Mouse (NA931) and Rabbit (NA934) IgG HRP-linked antibodies were purchased from GE Healthcare (Chicago, IL).
Rhodamine Phalloidin (PHDR1) was from Cytoskeleton (Denver, CO). Epidermal Growth Factor (EGF; cat. 236-EG) was purchased from R&D Systems. Gefitinib (ZD1839) was from Cayman Chemical Company (Ann Arbor, MI). AG-1478 (A8357) and MG-132 (A2585) were from ApexBio (Houston, TX). Cycloheximide (C1988) and leptomycin B (L2913) were purchased from Sigma-Aldrich.

Bahl S., Ling H., Acharige N.P., Santos-Barriopedro I., Pflum M.K, & Seto E. (2021). EGFR phosphorylates HDAC1 to regulate its expression and anti-apoptotic function. Cell Death & Disease, 12(5), 469.

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Dissecting Cellular Signaling Pathways

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Polyclonal goat anti-Septin4 (abcam, USA), polyclonal rabbit anti-HIF-1α (abcam), polyclonal rabbit anti-VHL (proteintech, USA), polyclonal rabbit anti-caspase-3 (Cell Signaling Technology, USA), monoclonal mouse anti-HA (Cell Signaling Technology), monoclonal mouse anti-Flag (abcam), and monoclonal mouse anti-GAPDH (abcam). CHX (A8244) and MG132 (A2585), used in DMSO, were purchased from ApexBio (USA). HIF-PHDs inhibitor BAY85-3934 was also purchased from ApexBio.

Wu S., Zhang Y., You S., Lu S., Zhang N., & Sun Y. (2020). Septin4 Aggravates Hypoxia-Induced Cardiomyocytes Injury by Promoting HIF-1α Ubiquitination and Degradation through VHL.

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