Plasma glucose was analyzed by enzymatic colorimetric assay (Glucose Oxidase kit; Instrumentation Laboratory, Warrington, United Kingdom) using an ILAB 650 Clinical Chemistry Analyzer (Instrumentation Laboratory). Plasma HbA1C concentration was analyzed via a Tosoh G8 HPLC semiautomated analyzer (Tosoh Bioscience, King of Prussia, PA). Serum interleukin-6 (IL-6), C-reactive protein (CRP), and insulin concentrations were measured using commercially available enzyme-linked immunosorbent assay kits (IL-6: R&D Systems Europe, Abingdon, United Kingdom; CRP: RayBiotech, Norcross, GA; insulin: IBL International, Hamburg, Germany).
Ilab 650 clinical chemistry analyzer
Manufactured by Werfen
Sourced in United Kingdom
The ILAB 650 is a clinical chemistry analyzer designed for the quantitative determination of various analytes in biological samples. It utilizes proven photometric and potentiometric detection principles to provide reliable and accurate results. The ILAB 650 is a compact and fully automated system suitable for routine use in clinical laboratories.
Automatically generated - may contain errors
2 protocols using ilab 650 clinical chemistry analyzer
1
Plasma Biomarker Quantification Protocol
2
Plasma Biomarkers in Blood Samples
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Venous blood samples (~6 mL) were collected into EDTA tubes, stored on ice and then centrifuged at 4°C and 1006 × g for 15 minutes. Aliquots of plasma were then stored at -70°C and later analysed for glucose (Glucose Oxidase kit; Instrumentation Laboratories, Cheshire, UK), NEFA (Randox, London, UK) and lactate (Randox, London, UK) using an ILAB 650 Clinical Chemistry Analyzer (Instrumentation Laboratory, Warrington, UK) and insulin using a commercially available ultrasensitive ELISA kit (Mercodia AB, Uppsala, Sweden). Area under the curve (AUC) was calculated between time points 'baseline' and 60-min of SS.
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