C3H10T1/2 cells were cultured in Roswell Park Memorial Institute 1640 medium (BioWest, Nuaille, France) supplemented with 10% foetal bovine serum and antibiotics (BioWest). 293T (human embryonic kidney cells) cells were maintained in Dulbecco's modified Eagle's medium (Corning, USA) supplemented with 10% foetal bovine serum and antibiotics. All cell lines were maintained at 37°C in a humidified atmosphere containing 5% CO2.
1640 medium
Manufactured by Biowest
Sourced in France
1640 medium is a cell culture medium that provides essential nutrients to support the growth and maintenance of a variety of cell types in vitro. It is a widely used basal medium that can be supplemented with additional components as required for specific cell line needs.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle s medium, by Corning (1 mentions)
Histopaque 1077, by Merck Group (1 mentions)
Ficoll hypaque density, by Merck Group (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Biowest (1 mentions)
Fgm 2 singlequot kit, by Lonza (1 mentions)
3 protocols using 1640 medium
1
Cell Culture Maintenance Conditions
2
Isolation of PBMCs from GBM Patients
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PBMCs were isolated from the blood of 14 patients with glioblastoma multiforme (GBM) and 14 age- and sex-matched healthy donors. Five milliliters of blood was collected in a sterile K3EDTA vacutainer tube. PBMCs were separated by differential centrifugation through a Ficoll–Hypaque density gradient (Histopaque-1077, Sigma-Aldrich, St. Louis, MO, USA), washed, and resuspended in 5% fetal bovine serum (FBS)/Roswell Park Memorial Institute (RPMI) 1640 medium (Biowest, Nuaillè, France). After the count, PBMCs were processed for analysis by immunofluorescence (see flow cytometry analysis paragraph for further details).
3
Culturing Normal Dermal Fibroblasts and Mouse Melanoma Cells
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Normal human dermal fibroblasts (NHDFs) were purchased from CELLnTEC (Switzerland) and grown in fibroblast growth media (FBM, Lonza, Switzerland) supplemented with an FGM-2 SingleQuot Kit (Lonza) at 37 °C with 5 % CO 2 . B16F10 mouse melanoma cells were grown in Roswell Park Memorial Institute 1640 medium (Biowest, France) supplemented with 10 % (v/v) fetal bovine serum (Biowest), 100 U mL -1 penicillin, and 100 μg mL -1 streptomycin (Gibco, USA) in a humidified 5 % CO 2 incubator at 37 °C.
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