Anti lamp1 clone h4a3

Manufactured by Santa Cruz Biotechnology

Anti-Lamp1 (clone H4A3) is a monoclonal antibody that recognizes the Lamp1 (Lysosome-associated membrane protein 1) protein. Lamp1 is a widely expressed lysosomal membrane protein. The antibody can be used for the detection of Lamp1 in various applications.

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Lab products found in correlation

Anti α tubulin clone b512, by Merck Group (1 mentions) Sc 6486, by Santa Cruz Biotechnology (1 mentions) Anti ubiquitin, by Agilent Technologies (1 mentions) Anti eea1 clone 14, by BD (1 mentions)

Spelling variants of this product

LAMP1 (83 citations) Anti-LAMP1 (46 citations) Anti-LAMP1 (H4A3) (7 citations)

2 protocols using anti lamp1 clone h4a3

Comprehensive Protein Detection Protocol

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Primary antibodies for western blotting (WB) and immunocytochemistry (ICC) were purchased and used at the indicated dilution; anti-LC3 (PM036, MBL; WB 1:3000; ICC 1:1000), anti-p62 (PM045, MBL; WB 1:5000), anti-Lamp1 (clone H4A3, sc-20011, Santa Cruz Biotechnology; ICC 1:1000), anti-EGFR (MI-12-1, MBL; WB 1:500), anti-cathepsin D (sc-6486, Santa Cruz Biotechnology; WB 1:1000), anti-EPG5 (sc-85198, Santa Cruz Biotechnology; WB 1:200), anti-GAPDH (MAb314, Chemicon; WB 1:5000), anti-α-tubulin (clone B-5-1-2, T5168, Sigma-Aldrich; WB 1:5000), anti-β-actin (clone 6D1, M177-3, MBL; WB 1:3000). BafA1 was used at 125 nM. PI comprised 10 μg/ml E64d and 10 μg/ml pepstatin A.

Hori I., Otomo T., Nakashima M., Miya F., Negishi Y., Shiraishi H., Nonoda Y., Magara S., Tohyama J., Okamoto N., Kumagai T., Shimoda K., Yukitake Y., Kajikawa D., Morio T., Hattori A., Nakagawa M., Ando N., Nishino I., Kato M., Tsunoda T., Saitsu H., Kanemura Y., Yamasaki M., Kosaki K., Matsumoto N., Yoshimori T, & Saitoh S. (2017). Defects in autophagosome-lysosome fusion underlie Vici syndrome, a neurodevelopmental disorder with multisystem involvement. Scientific Reports, 7, 3552.

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Immunostaining of Subcellular Markers

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Cells were immunostained as described previously (Hirota et al., 2004) . The following antibodies were used in this study: anti-AP-1 (clone 100/3, Sigma), anti-EEA1 (clone 14, BD Transduction Laboratories), anti-LAMP-1 (clone H4A3, Santa Cruz), anti-LC3 (MBL, #PM036), anti-p62 (MBL, #PM045), antiubiquitin (Dako, Z045801), and Alexa Fluor 589-labeled secondary antibodies (Molecular Probes). Immunofluorescence observations were performed using a laser-scanning fluorescence microscope (FV1000; Olympus) equipped with diode-pumped solid-state lasers (473 and 559 nm).

Tsutsui H., Jinno Y., Shoda K., Tomita A., Matsuda M., Yamashita E., Katayama H., Nakagawa A, & Miyawaki A. (2015). A diffraction-quality protein crystal processed as an autophagic cargo. Molecular cell, 58(1).

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