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Anti cd14 percp

Manufactured by BioLegend
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Anti-CD14-PerCP is a fluorescently conjugated monoclonal antibody that binds to the CD14 cell surface antigen, which is expressed on monocytes, macrophages, and some dendritic cells. The PerCP (Peridinin-Chlorophyll Protein Complex) fluorescent label allows for the detection and analysis of CD14-expressing cells using flow cytometry.

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Lab products found in correlation

Recombinant human macrophage colony stimulating factor m csf, by R&D Systems (1 mentions) Anti il 1β fitc, by BioLegend (1 mentions) Anti cd4 apc, by BioLegend (1 mentions) Anti cd11b apc cy7, by BioLegend (1 mentions) Anti cd16 fitc, by BioLegend (1 mentions) Pe conjugated streptavidin, by BioLegend (1 mentions) Facscanto 1, by BD (1 mentions) Fixable viability dye efluor 450, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Golgistop, by BD (1 mentions) Cytofix cytoperm kit, by BD (1 mentions) Anti cd8 pe cy7, by BioLegend (1 mentions) Anti cd19 pe cy7, by BioLegend (1 mentions) Anti cd127 pe cy7, by BioLegend (1 mentions) Anti tnfr2 apc, by R&D Systems (1 mentions) Anti cd25 fitc, by BioLegend (1 mentions) Anti cd3 pe cy7, by BioLegend (1 mentions) Anti cd4 pe cy7, by BioLegend (1 mentions) Anti tnfr2 pe, by R&D Systems (1 mentions) Anti cd45ra pacific blue, by BioLegend (1 mentions)

Close spelling variants of this product

PerCP anti-human CD14 Anti-CD14 PerCP/Cy5.5 Percp Cy5.5-anti-CD14 PerCP/Cyanine5.5 anti-mouse CD14 antibody CD14-PerCP

3 protocols using anti cd14 percp

1

Flow Cytometry Antibody Panel for Inflammatory Markers

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The antibodies for flow cytometry were anti-CD4-APC, anti-CD14-PerCP, and anti-IL-1β-FITC (Biolegend, CA, USA). Isotype-controlled mAbs were also purchased from Biolegend. Recombinant human macrophage colony stimulating factor (M-CSF) and the enzyme-linked immunosorbent assay (ELISA) kits for tumor necrosis factor-alpha (TNF-α), IL-1β, and active caspase-1 were purchased from R&D Systems. All other chemical reagents, unless otherwise specified, were purchased from Sigma-Aldrich (St. Louis, Mo).
Wu M.F., Shu C.C., Wang J.Y., Yan B.S., Lai H.C., Chiang B.L., Wu L.S, & Yu C.J. (2019). NLRP3 inflammasome is attenuated in patients with Mycobacterium avium complex lung disease and correlated with decreased interleukin-1β response and host susceptibility. Scientific Reports, 9, 12534.
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2

Flow Cytometric Analysis of IL-12 in PBMCs

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2.5 × 105 PBMCs were seeded in 96-well plates with RPMI 1640 (Gibco Cat # 11875119) supplemented with 10% FBS and 1% PS (Sigma Aldrich) and incubated with monensin (Golgistop; BD Biosciences Cat # 554715) for 5 h. After that, PBMCs were stained with anti-CD11b APC/Cy7 (BioLegend Cat # 10,225, RRID: AB_830641) anti-CD14-PerCP (BioLegend Cat # 325631, RRID: AB_2563327), anti-CD16-FITC (BioLegend Cat # 302005, RRID: AB_314205). Live cells were distinguished using Fixable Viability Dye eFluor™ 450 (eBioscience Cat # 65-0863-14). After staining of surface markers, cells were fixed and made permeable according to the manufacturer's instructions BD Cytofix/Cytoperm Kit (BD Bioscience Cat # 554715). Then, the cells were stained with anti-human IL-12-biotin (ThermoFisher Cat # AHC7129, RRID: AB_2536290) plus PE-conjugated Streptavidin (BioLegend Cat #405203), and acquired using a FACS Canto I (Becton Dickinson). All analysis was carried out with FlowJo software (Tree Star).
Nowak W., Grendas L.N., Sanmarco L.M., Estecho I.G., Arena Á.R., Eberhardt N., Rodante D.E., Aoki M.P., Daray F.M., Carrera Silva E.A, & Errasti A.E. (2019). Pro-inflammatory monocyte profile in patients with major depressive disorder and suicide behaviour and how ketamine induces anti-inflammatory M2 macrophages by NMDAR and mTOR. EBioMedicine, 50, 290-305.
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3

Phenotypic Immune Cell Analysis by Flow Cytometry

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Phenotypic characteristics of immune cells were assessed by flow cytometry (FACSVerse cytometer; BD, USA) using monoclonal antibodies: anti-CD25-FITC, anti-CD45R0-FITC, anti-CD19-PE-Cy7, anti-CD4-PE-Cy7, anti-CD3-PE-Cy7, anti-CD8-PE-Cy7, anti-CD127-PE-Cy7, anti-CD14-PerCP, and anti-CD45RA-Pacific Blue (Biolegend, USA); and anti-TNFR1-PE, anti-TNFR2-PE, anti-TNFR1-APC, and anti-TNFR2-APC (R&D Systems, USA). Data processing and calculation of fluorescence intensity parameters were performed in the FacsDiva software (BD, USA). To calculate the number of receptor molecules per cell, the BD QuantiBRITE PE Kit (BD Biosciences, USA) was used according to the method described earlier [22 (link)].
Alshevskaya A., Lopatnikova J., Zhukova J., Chumasova O., Shkaruba N., Sizikov A., Evsegneeva I., Demina D., Nepomniashchikch V., Karaulov A, & Sennikov S. (2022). The Influence of Severity and Disease Duration on TNF Receptors’ Redistribution in Asthma and Rheumatoid Arthritis. Cells, 12(1), 5.
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