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H 6023

Manufactured by Cell Biologics
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Sourced in United States

The H-6023 is a laboratory incubator designed for culturing cells. It maintains a controlled environment with temperature, humidity, and gas settings to support cell growth.

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Lab products found in correlation

Endogro mv complete media, by Merck Group (2 mentions) Scc0066, by Merck Group (2 mentions) C57 6023, by Cell Biologics (2 mentions) Enspire plate reader, by PerkinElmer (2 mentions) H1168, by Cell Biologics (1 mentions)

3 protocols using h 6023

1

AAV transduction efficiency in brain endothelial cells

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Multiple lots of mBMVEC and hBMVEC cells were obtained from CellBiologics (H-6023 & C57-6023) and maintained in endothelial cell media (H1168 & M1168). hCMEC/D3 cells were obtained from Millipore (SCC0066) and maintained in EndoGRO™-MV Complete Media (SCME004). All cells were handled according to the manufacturer’s instructions. For the Luciferase assays, 5000 cells/well were seeded in 96 well plates (PerkinElmer, 6005680). One day later, AAV9, AAV-PHP.eB or AAV-BI30 carrying pAAV-CAG-eGFP-p2A-luciferase was added at 20,000 vg/cell. 24 hours after transduction, a luciferase reporter assay was performed according to the manufacturer’s instructions (PerkinElmer, 6066761) on an EnSpire plate reader (PerkinElmer). For flow cytometry, hCMEC/D3 cells were plated at 434,000 cells/well in 24 well plates and exposed to the indicated dose of AAV-BI30 or AAV9. The media was exchanged for fresh media after 24 hours and transduction was assessed at 4 days post-administration on a Beckman CytoFLEX S Flow Cytometer using FlowJo 10.8.1 (BD Biosciences).
Krolak T., Chan K.Y., Kaplan L., Huang Q., Wu J., Zheng Q., Kozareva V., Beddow T., Tobey I.G., Pacouret S., Chen A.T., Chan Y.A., Ryvkin D., Gu C, & Deverman B.E. (2022). A High-Efficiency AAV for Endothelial Cell Transduction Throughout the Central Nervous System. Nature cardiovascular research, 1(4), 389-400.
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2

Treating HBEC with Tau Oligomers

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Primary human brain microvascular endothelial cells (HBEC) purchased from Cell Biologics (H-6023, Chicago, IL, USA) were cultured on gelatin-coated plates with complete human endothelial cell medium (Cell Biologics H1168, Chicago, IL, USA) in a 5% CO2 humidified incubator at 37 °C. All cells were plated at the same density prior to treatment. HBEC were treated with treated with 40 μg/mL tau oligomers (O. Tau), 40 μg/mL monomeric tau (M. tau), 40 μg/mL recombinant human cytokeratin-8 (KRT8, RayBiotech, Norcross, GA, USA), or vehicle (PBS) in 1 mL of complete media in a 35 mm plate for 42 h on a rocker (15 RPM) to simulate bidirectional low wall shear stress.
Hussong S.A., Banh A.Q., Van Skike C.E., Dorigatti A.O., Hernandez S.F., Hart M.J., Ferran B., Makhlouf H., Gaczynska M., Osmulski P.A., McAllen S.A., Dineley K.T., Ungvari Z., Perez V.I., Kayed R, & Galvan V. (2023). Soluble pathogenic tau enters brain vascular endothelial cells and drives cellular senescence and brain microvascular dysfunction in a mouse model of tauopathy. Nature Communications, 14, 2367.
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3

AAV transduction efficiency in brain endothelial cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Multiple lots of mBMVEC and hBMVEC cells were obtained from CellBiologics (H-6023 & C57-6023) and maintained in endothelial cell media (H1168 & M1168). hCMEC/D3 cells were obtained from Millipore (SCC0066) and maintained in EndoGRO™-MV Complete Media (SCME004). All cells were handled according to the manufacturer’s instructions. For the Luciferase assays, 5000 cells/well were seeded in 96 well plates (PerkinElmer, 6005680). One day later, AAV9, AAV-PHP.eB or AAV-BI30 carrying pAAV-CAG-eGFP-p2A-luciferase was added at 20,000 vg/cell. 24 hours after transduction, a luciferase reporter assay was performed according to the manufacturer’s instructions (PerkinElmer, 6066761) on an EnSpire plate reader (PerkinElmer). For flow cytometry, hCMEC/D3 cells were plated at 434,000 cells/well in 24 well plates and exposed to the indicated dose of AAV-BI30 or AAV9. The media was exchanged for fresh media after 24 hours and transduction was assessed at 4 days post-administration on a Beckman CytoFLEX S Flow Cytometer using FlowJo 10.8.1 (BD Biosciences).
Krolak T., Chan K.Y., Kaplan L., Huang Q., Wu J., Zheng Q., Kozareva V., Beddow T., Tobey I.G., Pacouret S., Chen A.T., Chan Y.A., Ryvkin D., Gu C, & Deverman B.E. (2022). A High-Efficiency AAV for Endothelial Cell Transduction Throughout the Central Nervous System. Nature cardiovascular research, 1(4), 389-400.
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