The comet assay was performed using method described by Li et al. [24] (link) and Singh et al. [35] (link). Comet assay was performed using the fresh blood sample according to the protocol. In order to minimize the variation, we placed the same numbers of samples collected from different groups (control, bottom, side and top groups) in one slide (CometSlide HT, Trevigen, USA). Analyses of the images were performed using Comet Assay Software Project-1.2.2 (University of Wroclaw, Poland). Olive tail moment (OTM) was selected as the parameter to indicate the degree of DNA damage.
Cometslide ht
Manufactured by Bio-Techne
Sourced in United States
The CometSlide HT is a laboratory equipment designed for the comet assay, also known as the single-cell gel electrophoresis assay. It is used to measure DNA damage and repair in individual cells. The CometSlide HT provides a consistent and standardized platform for performing the comet assay.
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Lab products found in correlation
2 protocols using cometslide ht
1
Comet Assay of DNA Damage
2
Evaluating DNA Damage in Lymphocytes
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Single cell gel electrophoresis (comet assay) was used to measure DNA strand breaks and alkaline labile sites in lymphocytes. The assay was conducted under alkaline conditions as previously described [30, 31] with slight modification for use with a high throughput CometSlide HT (Trevigen Inc. Cat 4252-02K-01). 100 cells were randomly selected from each spot and scored with online software (Tritek -http://autocomet.com/main_home.php) for tail moment and tail intensity. Tail moment (tail length X DNA density) and tail intensity (% DNA in tail) were used as indicators of DNA damage. Molecular Nutrition & Food Research This article is protected by copyright. All rights reserved.
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