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Biosystems viia 7 real time pcr system

Manufactured by Thermo Fisher Scientific
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The Biosystems ViiA 7 Real-Time PCR System is a laboratory instrument used for real-time polymerase chain reaction (PCR) analysis. It performs quantitative and qualitative nucleic acid detection and analysis.

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Lab products found in correlation

Quantifast sybr green pcr kit, by Qiagen (2 mentions) Mirvana mirna isolation kit, by Thermo Fisher Scientific (2 mentions) Superscript 3 reverse transcriptase system, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Real-Time System (23 citations) Applied Biosystem ViiA 7 Real-Time PCR system (5 citations) ViiA Real Time PCR (2 citations)

2 protocols using biosystems viia 7 real time pcr system

1

Quantitative Analysis of mRNA Levels

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Total RNA was extracted from cells (8 × 106 cells per replicate) using TRIzol Reagent followed by purification using the mirVana miRNA Isolation Kit (Thermo Scientific). cDNA was synthesized from 1 µg of purified RNA using SuperScript III reverse transcriptase system (Invitrogen) following the manufacturer’s protocol. qPCR was performed using the QuantiFast SYBR Green PCR kit (QIAGEN) with a Biosystems ViiA 7 Real-Time PCR System (Life Technologies). The housekeeping gene GAPDH was used as an internal control to normalize the variability in expression levels. The ΔΔCt relative quantification method was performed to measure relative quantitation of mRNA.
Huang H., Gont A., Kee L., Dries R., Pfeifer K., Sharma B., Debruyne D.N., Harlow M., Sengupta S., Guan J., Yeung C.M., Wang W., Hallberg B., Palmer R.H., Irwin M.S, & George R.E. (2021). Extracellular domain shedding of the ALK receptor mediates neuroblastoma cell migration. Cell reports, 36(2), 109363.
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2

Quantitative Analysis of mRNA Levels

Check if the same lab product or an alternative is used in the 5 most similar protocols
Total RNA was extracted from cells (8 × 106 cells per replicate) using TRIzol Reagent followed by purification using the mirVana miRNA Isolation Kit (Thermo Scientific). cDNA was synthesized from 1 µg of purified RNA using SuperScript III reverse transcriptase system (Invitrogen) following the manufacturer’s protocol. qPCR was performed using the QuantiFast SYBR Green PCR kit (QIAGEN) with a Biosystems ViiA 7 Real-Time PCR System (Life Technologies). The housekeeping gene GAPDH was used as an internal control to normalize the variability in expression levels. The ΔΔCt relative quantification method was performed to measure relative quantitation of mRNA.
Huang H., Gont A., Kee L., Dries R., Pfeifer K., Sharma B., Debruyne D.N., Harlow M., Sengupta S., Guan J., Yeung C.M., Wang W., Hallberg B., Palmer R.H., Irwin M.S, & George R.E. (2021). Extracellular domain shedding of the ALK receptor mediates neuroblastoma cell migration. Cell reports, 36(2), 109363.
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