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Bio plex protm rat cytokine 23 plex assay

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The Bio-Plex Pro Rat Cytokine 23-Plex Assay is a multiplex immunoassay designed to simultaneously detect and quantify 23 different rat cytokines in a single sample. The assay utilizes magnetic beads coated with capture antibodies specific to each target analyte. This technology enables the measurement of multiple cytokines from a small sample volume.

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Lab products found in correlation

Bio plex system, by Bio-Rad (1 mentions) Luminex 200 analyzer, by Hitachi (1 mentions) Masterplex ct control software, by Hitachi (1 mentions) Masterplex qt analysis software, by Hitachi (1 mentions)

4 protocols using bio plex protm rat cytokine 23 plex assay

1

Cytokine Profiling in Male Rats on HFD

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The cytokine profiles of male rats before and after HFD were assessed via Bio-Plex ProTM Rat Cytokine 23-Plex Assay (Bio-Rad Laboratories, Inc., Hercules, CA, USA, Luminex Corporation, Austin, TX, USA) for levels of G-CSF, GM-CSF, GRO/KC, IFN-γ, IL-1α, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-7, IL-10, IL-12p70, IL-13, IL-17, IL-18, M-CSF, MCP-1, MIP-1α, MIP-3α, RANTES, TNF-α, and VEGF using the BioPlex system (Bio-Rad Laboratories, Inc., Hercules, CA, USA).
Junková K., Mirchi L.F., Chylíková B., Janků M., Šilhavý J., Hüttl M., Marková I., Miklánková D., Včelák J., Malínská H., Pravenec M., Šeda O, & Liška F. (2021). Hepatic Transcriptome Profiling Reveals Lack of Acsm3 Expression in Polydactylous Rats with High-Fat Diet-Induced Hypertriglyceridemia and Visceral Fat Accumulation. Nutrients, 13(5), 1462.
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2

Cytokine Profiling of Microglia

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The microglial cell culture medium was collected and centrifuged for 20 min at 1000× g. Then, cytokine/chemokine concentrations in the supernatants were measured using a BioPlex ProTM Rat Cytokine 23-Plex Assay (Bio-Rad, Hercules, CA).
Aoki Y., Dai H., Furuta F., Akamatsu T., Oshima T., Takahashi N., Goto Y.I., Oka A, & Itoh M. (2023). LOX-1 mediates inflammatory activation of microglial cells through the p38-MAPK/NF-κB pathways under hypoxic-ischemic conditions. Cell Communication and Signaling : CCS, 21, 126.
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3

Rat Cytokine Profiling Assay

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Bio-Plex ProTM Rat Cytokine 23-Plex Assay (Cat# 12005641, BioRad) was used to determine the level of cytokines, chemokines, and growth factors in rat serum 24 and 48 hours after injection according to the manufacturer’s protocol and as described previously (Shaimardanova et al., 2022). Measurements were performed using a Luminex 200 analyzer with MasterPlex CT control software and MasterPlex QT analysis software (MiraiBio division of Hitachi Software, San Francisco, CA, USA).
Shaimardanova A.A., Chulpanova D.S., Solovyeva V.V., Issa S.S., Mullagulova A.I., Titova A.A., Mukhamedshina Y.O., Timofeeva A.V., Aimaletdinov A.M., Nigmetzyanov I.R, & Rizvanov A.A. (2023). Increasing β-hexosaminidase A activity using genetically modified mesenchymal stem cells. Neural Regeneration Research, 19(1), 212-219.
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4

Cytokine Profiling of Microglia

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The microglial cell culture medium was collected and centrifuged for 20 minutes at 1,000xg. Then, cytokine/chemokine concentrations in the supernatants were measured using a BioPlex ProTM Rat Cytokine 23-Plex Assay (BioRad, Hercules, CA).
Itoh M., Aoki Y., Akamatsu T., Oshima T., Takahashi N., Goto Y., & Oka A. (2020). LOX-1 Mediates Inflammatory Activation of Microglial Cells Through the p38-MAPK/NF-κB Pathways Under Hypoxic-Ischemic Conditions.
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