Samples in SDS Laemmli buffer were run on reducing SDS PAGE, either 10% Tris-glycine or Any kD TGX gels (Bio-Rad). Upon transfer to low fluorescence PVDF (Immobilon-FL, Millipore; or Immun-Blot LF, Bio-Rad), the membranes were incubated in Odyssey blocking buffer (Li-Cor Biosciences), then incubated with primary antibodies, followed by fluorescent secondary antibodies. For imaging on a Li-Cor Odyssey imaging system, the secondary antibodies were goat IRDye 800CW or 680LT antibodies (Li-Cor Biosciences). For imaging on a Bio-Rad ChemiDoc MP imaging system, the secondary antibodies were goat Alexa Fluor 647 or 546 antibodies (Invitrogen). The following primary antibodies were used: rabbit anti-NiV-M [50 (link)], rabbit anti-NiV-C (produced by 21st Century Biochemicals from rabbits immunized with a purified peptide corresponding to amino acids 13–31 of NiV-C), rabbit anti-HA (Novus, NB600-363), rabbit anti-COX IV (Li-cor Biosciences, 926–42214), mouse anti-Tsg101 clone 4A10 (Genetex, GTX70255), and mouse anti-FLAG clone M2 (Sigma, F3165).
Mouse anti tsg101 clone 4a10
Manufactured by GeneTex
Mouse anti-Tsg101 clone 4A10 is a primary antibody that recognizes the Tsg101 protein. Tsg101 is a key component of the endosomal sorting complex required for transport (ESCRT) machinery and plays a role in membrane trafficking and protein sorting.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti flag clone m2, by Merck Group (1 mentions)
Odyssey blocking buffer, by LI COR (1 mentions)
Immobilon fl, by Merck Group (1 mentions)
Chemidoc mp, by Bio-Rad (1 mentions)
Any kd tgx gels, by Bio-Rad (1 mentions)
Mouse anti flotillin 1, by BD (1 mentions)
Ab68418, by Abcam (1 mentions)
Ab134045, by Abcam (1 mentions)
Ab8219, by Abcam (1 mentions)
Ab38898, by Abcam (1 mentions)
2 protocols using mouse anti tsg101 clone 4a10
1
Western Blot Analysis of Virus Proteins
2
Antibody Panel for Endocytic Pathway
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Antibodies used were mouse anti-TSG101 (clone 4a10; GeneTex), mouse anti-Flotillin1 (610820; BD), mouse anticortactin (4F11; EMD Millipore), rabbit anti-CD63 for WB (ab68418 or ab134045; Abcam) and mouse anti-CD63 for immunofluorescence or endocytosis-EM studies (ab8219; Abcam), mouse anti–coronin 1b for WB (ab119071; Abcam), rabbit anti–coronin 1b for IF (gift from J.E. Bear, University of North Carolina Chapel Hill, Chapel Hill, NC), rabbit anti-EGFR (1005: sc-03; Santa Cruz Biotechnology, Inc.), rabbit anti-MMP9 (ab38898; Abcam), rabbit anti-Rab27 for WB (r4655; Sigma-Aldrich), mouse anti-MMP2 (F68; Daiichi Fine Chemical), rabbit collagen type 1 (600-406-103; Rockland), and mouse anti-MT1-MMP (clone ID8; Mori et al., 2002 (link)).
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