Annexin vpe apoptosis kit

All chemical reagents were purchased and used without further purification. Graphite powder, sulfuric acid, nitric acid, hydrogen peroxide, and sodium nitrate were purchased from Sinopharm Group Chemical Reagent Co., Ltd. (Guangzhou, China) Cisplatin and doxorubicin were purchased from Aladdin (Shanghai, China). 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N‐hydroxysuccinimide (NHS) were bought from Macklin Biochemical Technology Co., Ltd (Shanghai, China). RPMI 1640 medium, fetal bovine serum, trypsin, penicillin–streptomycin, and phosphate‐buffered saline were obtained from Gibco (California, USA). Cell counting kit‐8 (CCK‐8) was purchased from Beyotime Biotechnology (Shanghai, China). Annexin VPE apoptosis kit was bought from Becton, Dickinson, and Company (State of New Jersey, USA). GE11 peptide was synthesized by Sangon Biotech Co., Ltd (Shanghai, China). CNE‐2 (Nasopharyngeal carcinoma cell line) was supplied from Southern Medical University in Guangzhou.

The iCCA cells (3 × 10⁵/well) were cultured in six-well plates and were collected and washed twice with ice-cold PBS. Apoptosis was investigated by flow cytometry using the Annexin V-PE Apoptosis Kit (Becton Dickinson, San Diego, CA) following the protocol of the manufacturer.