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Ix71 research inverted system microscope

Manufactured by Olympus
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Sourced in Japan

The IX71 Research Inverted System Microscope is a high-performance inverted microscope designed for a variety of research applications. It features a stable and ergonomic design, a wide range of accessories, and advanced optical systems to deliver clear, high-quality images. The IX71 is capable of supporting a variety of observation techniques, including brightfield, phase contrast, and fluorescence microscopy.

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Lab products found in correlation

Alexa fluor 594 conjugated antibody, by Cell Signaling Technology (2 mentions) Dp73 17mp color camera, by Olympus (2 mentions) Lsm700 confocal microscope, by Olympus (2 mentions) Ham s f 12 media, by Merck Group (1 mentions)

Close spelling variants of this product

Research inverted system microscope IX71 inverted microscope Inverted research microscope IX71 microscope system Inverted system microscope IX71 microscope IX71 system Inverted microscope Olympus System Microscope

6 protocols using ix71 research inverted system microscope

1

Immunofluorescence Staining of Phosphorylated Histone H3

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Cytospin slides were fixed in 4% paraformaldehyde for 1 h, permeabilized in 0.25% Triton X-100 in PBS, blocked in 1% BSA and 2% FBS in PBS, followed by staining with AlexaFluor 488-conjugated anti-p-H3 antibody (22 (link)). Slides were mounted using Vectashield containing DAPI. Images were captured using a Zeiss LSM 700 confocal microscope or Olympus IX71 Research Inverted System Microscope with a DP73;17MP Color Camera.
For double staining for EdU and p-H3, cytospin slides were prepared after pulse labeling with EdU, followed by immunofluorescence staining for p-H3 using secondary AlexaFluor 594-conjugated antibody (Cell Signaling).
Zhou L., Zhang Y., Chen S., Kmieciak M., Leng Y., Lin H., Rizzo K.A., Dumur C.I., Ferreira-Gonzalez A., Dai Y, & Grant S. (2014). A regimen combining the Wee1 inhibitor AZD1775 with HDAC inhibitors targets human acute myeloid leukemia cells harboring various genetic mutations. Leukemia, 29(4), 807-818.
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2

Adhesion of H. pylori to AGS Cells

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AGS cells were seeded at 2 × 105 cells/well in 24-well plates containing Ham’s F-12 medium supplemented with 10% FBS under the standard cell culture conditions and allowed to adhere overnight. When AGS cells reached approximately 80% confluency, the medium was aspirated and the cells were washed twice each with 1 mL of PBS. Different strains of H. pylori suspension harvested at log phase of growth (OD600 ≌ 1.0) were added to AGS cells at a multiplicity of infection (MOI) of 100. After 4 hours of co-culture, the morphological changes of these cells were recorded by using a IX71 research inverted system microscope (Olympus; Japan) with 20 × magnification. For adhesion assay, bacteria were co-cultured with AGS cells for 4 or 6 hours. The non-adhered bacteria were removed by PBS buffer wash and then the cells were lysed by treating with 0.5% saponin-containing PBS buffer for 5 minutes of incubation. The obtained lysate was serially diluted and spread on sheep blood agar plates. The adhered bacteria were measured by the viable plate counting method after 72 to 96 hours of incubation.
Chiu S.F., Teng K.W., Wang P.C., Chung H.Y., Wang C.J., Cheng H.C, & Kao M.C. (2021). Helicobacter pylori GmhB enzyme involved in ADP-heptose biosynthesis pathway is essential for lipopolysaccharide biosynthesis and bacterial virulence. Virulence, 12(1), 1610-1628.
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3

Immunofluorescence Staining of Phosphorylated Histone H3

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Cytospin slides were fixed in 4% paraformaldehyde for 1 h, permeabilized in 0.25% Triton X-100 in PBS, blocked in 1% BSA and 2% FBS in PBS, followed by staining with AlexaFluor 488-conjugated anti-p-H3 antibody (22 (link)). Slides were mounted using Vectashield containing DAPI. Images were captured using a Zeiss LSM 700 confocal microscope or Olympus IX71 Research Inverted System Microscope with a DP73;17MP Color Camera.
For double staining for EdU and p-H3, cytospin slides were prepared after pulse labeling with EdU, followed by immunofluorescence staining for p-H3 using secondary AlexaFluor 594-conjugated antibody (Cell Signaling).
Zhou L., Zhang Y., Chen S., Kmieciak M., Leng Y., Lin H., Rizzo K.A., Dumur C.I., Ferreira-Gonzalez A., Dai Y, & Grant S. (2014). A regimen combining the Wee1 inhibitor AZD1775 with HDAC inhibitors targets human acute myeloid leukemia cells harboring various genetic mutations. Leukemia, 29(4), 807-818.
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4

H. pylori Infection of AGS Cells

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AGS cells (ATCC 1739, human gastric adenocarcinoma epithelial cell line) were seeded at 2 × 105 cells/well in 6 cm dishes containing Ham’s F-12 media (Sigma-Aldrich) supplemented with 10% FBS under standard cell culture conditions. When the AGS cells reached approximately 80% confluency, suspensions from different strains of H. pylori harvested at the log phase (OD 600 ≌ 1.0) were added to the AGS cells at a multiplicity of infection (MOI) of 100. After 6 h of co-culturing the morphological changes of the cells were recorded by using an Olympus IX71 research inverted system microscope (Olympus, Tokyo, Japan) with 20× magnification.
Liu A.N., Teng K.W., Chew Y., Wang P.C., Nguyen T.T, & Kao M.C. (2022). The Effects of HP0044 and HP1275 Knockout Mutations on the Structure and Function of Lipopolysaccharide in Helicobacter pylori Strain 26695. Biomedicines, 10(1), 145.
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5

Rhodamine-Labeled Polymer Cellular Uptake

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Rhodamine-labeled polymers were synthesized using methacryloxyethyl thiocarbamoyl rhodamine B (0.1 mol% of monomers), as described above. HeLa cells (1 × 105 cells) were seeded in a 12-well dish and pre-incubated for 24 h in DMEM in the presence of serum (10% FBS). Cells were washed twice with Hanks’ balanced salt solution (HBSS). Subsequently, 1 mL of fresh DMEM with serum was added to the dish along with the rhodamine-labeled polymer solution in PBS(−), and cells were incubated for 15 min at 37 °C and 5% CO2. The final polymer concentration was 1 mg/mL. After incubation, the cells were washed with HBSS three times, and 1 mL of HBSS was added to the dish. Cells were then observed under a fluorescence microscope (Research inverted system microscope IX71, Olympus, Tokyo, Japan).
Yoshizaki Y, & Konno T. (2023). Cellular Internalization and Exiting Behavior of Zwitterionic 4-Armed Star-Shaped Polymers. Molecules, 28(11), 4479.
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6

Immunofluorescence Analysis of Inflammasome Components

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After anesthetisation, the mice were transcardially perfused with normal saline (0.9 %), and brain tissues were fixed in a fresh 4 % paraformaldehyde solution (pH 7.4) at 4 °C. Coronal sections (30 μm) containing the diencephalon were prepared for immunofluorescence staining. Primary antibodies against NLRP3, caspase-1 and IL-1β were also used to delineate respective inflammasome components. DAPI (4′,6-diamidino-2-phenylindole) was used for nuclear staining. Alexa Fluor 488, Alexa Fluor 555 and Alexa Fluor 647 were used as secondary antibodies. Images of the stained specimens (five mice per group) were captured using an Olympus Research Inverted System Microscope IX71 (Olympus, Japan).
Zhang Z.T., Du X.M., Ma X.J., Zong Y., Chen J.K., Yu C.L., Liu Y.G., Chen Y.C., Zhao L.J, & Lu G.C. (2016). Activation of the NLRP3 inflammasome in lipopolysaccharide-induced mouse fatigue and its relevance to chronic fatigue syndrome. Journal of Neuroinflammation, 13, 71.
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