Mouse igg2a k

Manufactured by Thermo Fisher Scientific

Mouse IgG2a κ is an immunoglobulin class and subclass specific to the mouse species. It serves as a tool for research and scientific applications.

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Lab products found in correlation

Anti human cd14 phycoerythrin pe cy7, by Thermo Fisher Scientific (1 mentions) Clone rpa t4, by Thermo Fisher Scientific (1 mentions) Clone rpa t8, by Thermo Fisher Scientific (1 mentions) Anti human cd127 fitc, by Thermo Fisher Scientific (1 mentions) Anti human cd4 percp cy5, by BD (1 mentions) Clone m a251, by BD (1 mentions) Mouse igg1k, by Thermo Fisher Scientific (1 mentions) Anti human hla dr apc h7 clone g46 6, by Thermo Fisher Scientific (1 mentions) Mouse igg2aκ, by Thermo Fisher Scientific (1 mentions) Gallios cytometer, by Beckman Coulter (1 mentions)

Close spelling variants of this product

IgG2a (72 citations) Mouse IgG2a (14 citations)

2 protocols using mouse igg2a k

Immune Phenotyping of T Cells and Dendritic Cells in Kawasaki Disease

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Given the role of T cells and myeloid dendritic cells (DC) in the pro- and anti-inflammatory pathways in KD, we focused on immune phenotyping these cell populations. Myeloid DC populations were characterized and enumerated by flow cytometry using the following monoclonal antibodies (mAb) : anti-human CD11c-APC, mouse IgG1κ, clone B-ly6, anti-human CD11b-APC-Cyanin 7 (Cy7), mouse IgG1κ, clone ICRF44, anti-human CD14-phycoerythrin (PE) Cy7, mouse IgG2aκ, clone M5E2, anti-human CD86-fluorescein isothiocyanate (FITC), mouse IgG1κ, clone 2331 (FuN-1) (eBioscience).
T cell populations were characterized and enumerated by flow cytometry using the following mAb: CD25 BV421, mouse IgG1 k, clone M-A251 (BD Bioscience), anti-human CD4-percp-Cy5.5, mouse IgG1k, clone RPA-T4, anti-human CD8, Alexa Fluor 700, clone RPA-T8, mouse IgG1k, anti-human CD45RA APC, mouse IgG2b k, clone HI100, anti-human CD127 FITC, mouse IgG1 k, clone eBioRDR5, anti-human HLA-DR APC-H7 clone G46-6, mouse IgG2a k (eBioscience). Data were acquired with FACS ARIA II and analyzed using FACSDiva (BS Biosciences, San Jose, CA).

Tremoulet A.H., Jain S., Jone P.N., Best B.M., Duxbury E.H., Franco A., Printz B., Dominguez S.R., Heizer H., Anderson M.S., Glodé M.P., He F., Padilla R.L., Shimizu C., Bainto E., Pancheri J., Cohen H.J., Whitin J.C, & Burns J.C. (2019). Phase I/IIa Trial of Atorvastatin in Patients With Acute Kawasaki Disease With Coronary Artery Aneurysms. The Journal of pediatrics, 215, 107-117.e12.

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Flow Cytometry Analysis of Immune Cell TLR Expression

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For mouse primary hepatic macrophage and hepatocyte flow cytometry, 10⁵ cells were labeled with 4 μg/ml of isotype control antibody (rat IgG2a-PE, Cambridge Bioscience 400507, 1:50), rat anti-mouse TLR2-PE (Cambridge Bioscience, 148603, 1:50) or anti-TLR4-PE (Cambridge Bioscience, 145403, 1:50). For human monocyte, PBMC and HepG2 cell-line flow cytometry, antibodies used were AF488-labeled anti-TLR2, anti-TLR4 and mouse IgG2a-K isotype control (eBioscience, 53-9922-41, 1:20, 53-9917-41, 1:20 & 53-4724-80, 1:50). Data were acquired using a Gallios cytometer (Beckman Coulter).

Faraj T.A., Stover C, & Erridge C. (2019). Dietary Toll-Like Receptor Stimulants Promote Hepatic Inflammation and Impair Reverse Cholesterol Transport in Mice via Macrophage-Dependent Interleukin-1 Production. Frontiers in Immunology, 10, 1404.

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