A wound-healing assay was used to examine cell migration. Briefly, A549 and PC-9 cells were frequently cultivated until they reached confluence, replacing the medium with serum-free DMEM medium, making wounds with a 10-μL pipette tip, and capturing phase-contrast microscopic images. The capacity to invade cells was assessed through a transwell assay as outlined earlier. Briefly, in a room comprising an 8-μm polycarbonate filter (Corning Life Sciences, Corning, NY, USA) covered with 30 μL of Matrigel (Corning Life Sciences), LUAD cells in 300 μL of serum-free medium were grown. Cells remaining on the upper membrane were removed with a cotton swab after incubating for 24 h, and cells penetrating the membrane were fixed with 4% formaldehyde and then stained for 20 min with 0.5% crystal violet. All statistical results were acquired from an average of three autonomous studies from five picture areas chosen at random.
8 μm polycarbonate filter
Manufactured by Corning
Sourced in United States
The 8-μm polycarbonate filter is a laboratory equipment product designed for filtration applications. It features a pore size of 8 micrometers and is made of polycarbonate material. The core function of this filter is to separate and retain particles or materials of a specific size from a liquid or gas sample.
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Lab products found in correlation
2 protocols using 8 μm polycarbonate filter
1
Cell Migration and Invasion Assays
2
Transwell Assay for Cell Migration
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Cell migration was detected using a 24-well transwell plate (8 μm polycarbonate filter, Corning, USA, Cat 3422). Cells were seeded in the plate at a cell density of 3 × 105 cells/mL into the upper chamber with 100 μL per chamber, while 600 μL of DMEM containing 10% FBS was added into the lower chamber. After incubation for 24 hr, cells on the upper surfaces of upper chambers were removed with cotton-tipped swabs, and cells migrated to the lower surface were fixed in formaldehyde for 20 min and then stained with crystal violet staining solution (Beyotime, China, Cat Co121-100ml) and counted.
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