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2 protocols using stearic acid d3

1

Comprehensive Lipidomic and Metabolomic Protocol

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Ammonium acetate, LC-MS-grade formic acid (FA), isopropanol and acetonitrile, and HPLC-grade methanol and methyl tert-butyl ether (MTBE) were purchased from Fisher Scientific (Geel, Belgium). Lipidomics Isotope-labelled internal standards (13 (link)) and Lyso-phosphatidylcholine (LPC) 19:0 (for metabolomics analysis) were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Isotope-labeled internal standards for metabolomics analysis, including tryptophan-d5, valine-d8, phenylalanine-d5, palmitic acid-d3, cholic acid-d4, stearic acid-d3, carnitine C8:0-d3, and carnitine C16:0-d3, were obtained from Cambridge Isotope Laboratories (Tewksbury, MA, USA). Ultrapure water was prepared using the Milli-Q system (Millipore, Billerica, USA).
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2

Metabolite Profiling with Isotopically Labeled Standards

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Formic acid (FA), acetonitrile (ACN), and ammonium acetate (NH4OAc) were obtained from Thermo Fisher, United States. Tryptophan-d5, valine-d8, carnitine C8:0-d3, carnitine C16:0-d3, cholic acid-d4, and stearic acid-d3 (98% purity) were purchased from Cambridge Isotope Laboratories (Tewksbury, MA, United States). Lysophosphatidylcholine LPC (19:0) was obtained from Avanti Polar Lipids (Alabaster, AL, United States). Pure water was purchased from Wahaha ((Hangzhou, China).
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