Phospho specific akt thr308

Manufactured by Cell Signaling Technology

Sourced in United States

Phospho-specific Akt (Thr308) is an antibody product that specifically recognizes the phosphorylated form of the Akt protein at threonine 308. Akt is a key signaling molecule involved in various cellular processes. This antibody can be used to detect and analyze the activation state of Akt in biological samples.

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Lab products found in correlation

Ecl western blotting detection kit, by GE Healthcare (1 mentions) Ver 155008, by Merck Group (1 mentions) Phospho specific p44 p42 map kinase, by Cell Signaling Technology (1 mentions) P44 p42 map kinase, by Cell Signaling Technology (1 mentions) Akt antibody, by Cell Signaling Technology (1 mentions) Igf 1, by R&D Systems (1 mentions) Ym 08, by Merck Group (1 mentions) Glyceraldehyde 3 phosphate dehydrogenase gapdh antibody, by Santa Cruz Biotechnology (1 mentions)

2 protocols using phospho specific akt thr308

IGF-I Signaling Pathway Analysis

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IGF-I was obtained from R&D System, Inc. (Minneapolis, MN, USA). VER-155008 and YM-08 were obtained from Sigma-Aldrich Co. (St. Louis, MO, USA). Phospho-specific p44/p42 MAP kinase, p44/p42 MAP kinase, phospho-specific Akt (Thr308), and Akt antibodies were used for the first antibodies (Cell Signaling, Beverly, MA, USA). An ECL Western blotting detection kit was used (GE Healthcare UK Ltd., Buckinghamshire, UK). Other materials were purchased from commercial sources. VER-155008 and YM-08 were dissolved in dimethyl sulfoxide (DMSO). The maximum concentration of DMSO was 0.1%, which did not affect the assay for cell migration and the detection of the protein level using Western blotting.

Kawabata T., Tokuda H., Sakai G., Fujita K., Matsushima-Nishiwaki R., Kuroyanagi G., Otsuka T, & Kozawa O. (2018). HSP70 Inhibitor Suppresses IGF-I-Stimulated Migration of Osteoblasts through p44/p42 MAP Kinase. Biomedicines, 6(4), 109.

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Western Blot Antibody Reagents

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Phospho-specific Akt (Thr-308) and phospho-specific p38 mitogen-activated protein (MAP) kinase antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA). Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) antibody was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Other materials and chemicals were obtained from commercial sources.

Tokuda H., Hori T., Mizutani D., Hioki T., Kojima K., Onuma T., Enomoto Y., Doi T., Matsushima-Nishiwaki R., Ogura S., Iida H., Iwama T., Sakurai T, & Kozawa O. (2024). Inverse relationship between platelet Akt activity and hippocampal atrophy: A pilot case-control study in patients with diabetes mellitus. World Journal of Clinical Cases, 12(2), 302-313.

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