Bal tec cpd 030

Manufactured by Oerlikon Balzers

Sourced in Liechtenstein

The Bal-Tec CPD 030 is a critical point dryer used for the preparation of samples for scanning electron microscopy (SEM) and other high-resolution imaging techniques. It is designed to gently remove the liquid from the sample while preserving its structure and morphology.

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Lab products found in correlation

Bal tec scd 005, by Oerlikon Balzers (2 mentions) Vega lmu, by TESCAN (1 mentions) Jsm 7500f, by JEOL (1 mentions)

Spelling variants of this product

CPD 030 (72 citations) BAL-TEC (7 citations) Bal-Tec model cpd 030 Drier (2 citations)

3 protocols using bal tec cpd 030

Scanning Electron Microscopy of Floral Structures

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Fragments of the corolla petals and calyx as well as stamens were fixed in a 4% glutaraldehyde solution in 0.1 M phosphate buffer (pH 7.0). After 12 h incubation at a temperature of 4 °C, the samples were washed in the same buffer four times at 20 min intervals. An ethanol series (30, 50, 70, 90, and 95%) was used to dehydrate the plant material. Next, the samples were immersed three times in absolute alcohol and transferred to acetone. The samples were critical point dried in liquid CO₂ using Bal-Tec CPD 030 (Balzers, Liechtenstein).
Dried fragments of the examined floral parts were glued onto stubs with the use of a double-sided carbon tape. The samples were coated with a 10 μm gold layer using a Polaron SC 7640 sputter coater (Emitech). A scanning electron microscope TESCAN/VEGA LMU (Tescan) at an accelerating voltage of 30 kV was used for the examination of the material.

Sulborska A., Konarska A., Matysik-Woźniak A., Dmitruk M., Weryszko-Chmielewska E., Skalska-Kamińska A, & Rejdak R. (2020). Phenolic Constituents of Lamium album L. subsp. album Flowers: Anatomical, Histochemical, and Phytochemical Study. Molecules, 25(24), 6025.

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Sample Preparation for FESEM Imaging

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Prior to proceed with FESEM processing, cover slips were coated with poly-L-lysin and left to air-dry. After post fixation and en bloc, the fixatives were removed completely and rinsed with 0.1 M PBS, then proceed with dehydration phase using graded series of ethanol (30 %, 50 %, 70 %, 90 %, and 100 % for three times) for 5 mins respectively, thus drop 100 μl of the samples on the coated cover slips. Sample was then transferred to a gasket and need to be in 100 % ethanol before putting it into the critical point dryer (Baltec CPD-030, Balzers, Liechtenstein) to remove all the extracellular fluid and at the same time maintaining the intracellular fluid of the sample. For critical point drying with carbon dioxide (CO₂), the gasket was put in the chamber when the chamber was cooled to 10 °C and followed by sequence of medium in and out to remove the ethanol presence in the chamber, this sequence was repeated 6 times. Then, the chamber was heated into 42 °C for 2 h and ensured the pressure was above 85 bar. After drying and gas out the chamber, the sample was put on the stub and sprayed with platinum layers for sample conductivity using Sputter coater (Baltec SCD-005, Balzers, Liechtenstein). The sample was then ready to be viewed under FESEM (Teneo FEI, USA).

Muhamad Hendri N.A., Nor Amdan N.A., Dounis S.O., Sulaiman Najib N, & Louis S.R. (2024). Ultrastructural and morphological studies on variables affecting Escherichia coli with selected commercial antibiotics. The Cell Surface, 11, 100120.

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SEM Analysis of Polymer Biofilms

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Strips or fragments of subsamples of two replicates (out of five) of each synthetic polymer and glass were fixed at 4 °C in sterile sea water containing 2.5% glutaraldehyde and 50 mM sodium cacodylate (pH 7.2) and stored at 4 °C (4-10 days) until processing. Before, one subsample of each replicate (n = 2) was washed to remove the unspecific loosely attached part of the biofilm as described above; the other one remained untreated to visualize the whole community. Samples were stepwise dehydrated in ethanol, critical point dried (BAL-TEC CPD 030; Balzers, Liechtenstein) and sputter coated (BAL-TEC SCD 005; Balzers, Liechtenstein) with gold-palladium before SEM analysis (JEOL JSM-7500F; Freising, Germany).

Kirstein I.V., Wichels A., Krohne G, & Gerdts G. (2018). Mature biofilm communities on synthetic polymers in seawater - Specific or general?. Marine environmental research, 142.

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