Eca109

Human esophageal epithelial cell line (HEEC) and primary EC cell lines (ECA109, KYSE410, KYSE150, TE1) were purchased from BioVector NTCC Inc. (Beijing, China). These EC cell lines are from the same histological type (squamous cell carcinoma). Roswell Park Memorial Institute-1640 (RPMI-1640; Gibco, Carlsbad, CA, USA) containing 10% fetal bovine serum (Gibco) and 1% antibiotics (Gibco) was used for cell culture in a 5% CO₂ incubator at 37 °C. Small interfering RNA (siRNA) against circ-SFMBT2 or SLC1A5 (si-circ-SFMBT2, si-SLC1A5), mimic or inhibitor for miR-107 (miR-107, anti-miR-107) and the negative controls (si-NC, si-con, miR-NC, anti-miR-NC) were directly bought from GenePharma (Shanghai, China). The pCD5-ciR, pCD5-ciR-circ-SFMBT2 (circ-SFMBT2), pcDNA and pcDNA-SLC1A5 (SLC1A5) plasmids were provided by GENESEED (Guangzhou, China). KYSE150 and TE1 cells were transfected with RNAs (40 nM siRNA, 40 nM mimic, 20 nM inhibitor) or 2 μg plasmids by Lipofectamine™ 3000 (Invitrogen, Carlsbad, CA, USA).

Human esophageal epithelial cells (HEEC) and human EC cell lines (KYSE-520 and ECA-109) were purchased from BioVector NTCC Inc. (Beijing, China). These cells were cultured with RMPI-1640 culture medium (Gibco BRL, Paisley, UK) containing 10% fetal bovine serum (FBS) in a humidified incubator (37°C, 5% CO₂) [32 (link)]. Cells were passage when the confluence until to 80%.