Rhodamine 123 solution

Manufactured by Merck Group

Sourced in United States

Rhodamine-123 solution is a fluorescent dye used in various laboratory applications. It is a water-soluble, cationic dye that exhibits green fluorescence when excited by light. Rhodamine-123 is commonly used as a marker for mitochondria in live cells, as it selectively accumulates in these organelles.

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Rhodamine 123 (453 citations) Rhodamine solution (3 citations)

3 protocols using rhodamine 123 solution

Rhodamine-123 and PI Staining for Cell Viability

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RAW 264.7 cells (1×10⁶ cells/ml) were incubated with reagents at 37℃ for 10~12 h. The cells were harvested, washed in ice-cold PBS, and resuspended in 500µl of 4µg/ml rhodamine-123 solution (Sigma-Aldrich) at 37℃ for 20 min. The rhodamine-123-labeled cells were then incubated with 500µl of 0.1 mg/ml PI solution for 3 min. Incorporation of rhodamine-123 and PI was analyzed using the FACSCalibur flow cytometer. The fluorescence of rhodamine-123 (green) and PI (red) were detected using FL1 and FL2 sensors, respectively. All data were analyzed using Cell Quest software.

Kim Y.C., Song S.B., Lee S.K., Park S.M, & Kim Y.S. (2014). The Nuclear Orphan Receptor NR4A1 is Involved in the Apoptotic Pathway Induced by LPS and Simvastatin in RAW 264.7 Macrophages. Immune Network, 14(2), 116-122.

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Rhodamine 123 Staining of Ti(OH)4-Treated MB49 Cells

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MB49 cells (1 × 10⁴ cells/mL) were
seeded in coverslips placed on six-well plates and treated with one
dose of Ti(OH)₄ at a concentration of 6.0 μg/mL.
Cells were washed with DMEM, and 50 μL of rhodamine 123 solution
(1 mg/mL in ethanol, Sigma-Aldrich, USA) was added for 15 min in the
dark at 37 °C. Afterward, cells were washed with PBS (1×),
and coverslips were assembled on slides for observation under a fluorescence
microscope. Images were captured at 40× magnification and analyzed
with an ImageJ software program.^{81 (link)}

Robeldo T., Ribeiro L.S., Manrique L., Kubo A.M., Longo E., Camargo E.R, & Borra R.C. (2022). Modified Titanium Dioxide as a Potential Visible-Light-Activated Photosensitizer for Bladder Cancer Treatment. ACS Omega, 7(21), 17563-17574.

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Hydrogel Imaging Using SEM and Confocal Microscopy

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Imaging of dry hydrogel specimens was obtained using a scanning electron microscope (SEM) (Quanta 200 Scanning Electron Microscope – FE – operating mode: low vacuum, gaseous secondary electron GSE detector). Hydrated silk hydrogels were first quenched in liquid nitrogen and subsequently lyophilized for 48 hours to prepare dry cross sections for imaging. Prior to imaging, lyophilized cross sections were sputter coated (Biorad SC500, Hemel Hempstead, UK) with a thin layer of gold to avoid charging of the sample.
Imaging of wet hydrogel specimens was performed by submerging neat SF hydrogel matrices in 0.1 mg/ml Rhodamine123 solution (Sigma) at 4 °C for 8 hours (Rh123 is a greenfluorescent small molecule excitation 485nm, emission 535 nm) and then repeatedly washed in DI water to remove all un-bound dye. The non-specific adsorption of Rh123 within the SF matrices allows for visual observation of the wet hydrogel morphology [36 (link)]. Gels were then placed on glass slides for confocal imaging. Samples were imaged with Argon-ion laser at 488nm coupled with a band-pass emission filter 535/15 nm using a confocal microscope Nikon A1 model.

Floren M., Bonani W., Dharmarajan A., Motta A., Migliaresi C, & Tan W. (2015). Human Mesenchymal Stem Cells Cultured on Silk Hydrogels with Variable Stiffness and Growth Factor Differentiate into Mature Smooth Muscle Cell Phenotype. Acta biomaterialia, 31, 156-166.

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