Mouse monoclonal anti-Cav1 (Cat. #: 610406, clone name: 2297/Caveolin 1) and anti-Cav3 (Cat. #: 610420, clone name: 26/Caveolin 3) antibodies were from BD Transduction Laboratories. Mouse monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody (Cat. #: MAB374, clone name: 6C5) was from Merck Millipore. Rabbit polyclonal anti-PTRF/Cavin-1 (Cat. #: ab48824) and anti-SRBC/Cavin-3 (Cat. #: 16250-1-AP) antibodies were from Abcam and Proteintech, respectively. Rabbit polyclonal anti-MURC/Cavin-4 antibody was generated as previously described [27 (link),28 (link)]. Rabbit monoclonal anti-phospho-ERK1/2 (pERK, Cat. #: 9101S), rabbit monoclonal anti-ERK (Cat. #: 9102S), rabbit polyclonal anti-phospho-Akt (pAkt, Cat. #: 9271S), rabbit polyclonal anti-Akt (Cat. #: 9272S), rabbit polyclonal anti-phospho-JNK (pJNK, Cat. #: 9251S), rabbit polyclonal anti-JNK (Cat. #: 9252S), mouse monoclonal anti-phospho-p38 (pp38, Cat. #: 9216S, clone name: 28B10), and rabbit polyclonal anti-p38 (Cat. #: 9212) antibodies were from Cell Signaling Technology, Inc. Other materials were obtained from commercial sources.
Mouse monoclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh antibody
Manufactured by Merck Group
Sourced in United States
The mouse monoclonal anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) antibody is a laboratory reagent used in various immunoassays and research applications. GAPDH is a housekeeping gene that is constitutively expressed in most cell types and is often used as a control or reference protein in experiments.
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2 protocols using mouse monoclonal anti glyceraldehyde 3 phosphate dehydrogenase gapdh antibody
1
Antibody Sources for Caveolins and Signaling Proteins
2
Immunoblotting of Mitochondrial Proteins
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Immunoprecipitation, sodium dodecyl sulfate polyacrylamide gel electrophoresis and immunoblotting was carried out as previously described5 . Primary antibodies used were mouse monoclonal anti‐Src antibody from Merck KGaA (Darmstadt, Germany); mouse monoclonal anti‐complex I (39 kDa subunit), anti‐complex III (core II), anti‐complex IV (subunit I) and anti‐complex V (subunit α) of mitochondrial respiratory chain antibody from Invitrogen (Eugene, OR, USA); rabbit anti‐glucokinase antibody and anti‐GLUT2 antibody from Abcam (Cambridge, UK); rabbit anti‐Lyn, anti‐Lck, anti‐Fgr, anti‐Blk, anti‐C‐terminal Src kinase (Csk), anti‐calnexin and goat anti‐Hck antibody from Santa Cruz Biotechnology (Santa Cruz, CA, USA); rabbit anti‐neuronal nitric oxide synthase (nNOS) antibody from Cell Signaling Technology (Danvers, MA, USA); mouse monoclonal anti‐β‐actin antibody from Sigma‐Aldrich; and mouse monoclonal anti‐glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) antibody from Merck KGaA. Secondary antibodies used were horseradish peroxidase‐conjugated anti‐rabbit and mouse antibody from GE Healthcare (Buckinghamshire, UK), and anti‐goat antibody from Merck KGaA. Band intensities were quantified with Multi Gauge software (Fuji Film, Tokyo, Japan).
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