Ix2 rfaca fluorescent microscope

Rhodamine-DOPE was introduced for lipid composition labeling to evaluate cellular uptake. U251-CD133⁺ cells, U251-CD133^– cells and BCECs were seeded at a density of 5 × 10⁴ cells/well in six-well plates. After 48 h incubation in their own respective culture conditions, cells were examined for confluency and morphology using the light microscopy. They were then incubated with either DP-CLPs–PTX–survivin siRNA, CLPs–PTX–survivin siRNA and the control (DP-CLPs–PTX–scrambled siRNA and the CLPs–PTX–scrambled siRNA) at quantities required to deliver 10 μg of lipids per well in the presence of serum-free medium for 60 min at 37 °C. Cells were then washed with Hanks’ balanced salt solution and visualized and photographed using an IX2-RFACA fluorescent microscope (Olympus, Osaka, Japan).