Uas sce rnai

Flies were maintained on standard cornmeal/agar food at 25°C, unless otherwise noted. For RNAi experiments, flies were incubated on standard cornmeal/agar food at 29°C for 5-7 days prior to dissection and imaging to boost Gal4 activity unless otherwise noted.
The following fly strains were used in this study: w¹¹¹⁸, VCP-GFP (Wall et al., 2021 (link)), UAS-VCP-RNAi (Vienna Drosophila Resource Center, #24354; FBst0455418), BamGal4 (Doug Harrison, University of Kentucky, KY, USA), sa-GFP (Chen et al., 2005 (link)), UAS-bam-RNAi [Bloomington Drosophila Stock Center (BDSC), #33631; FBst0033631), UAS-sa-RNAi (BDSC, #36730; FBst0036730), UAS-mia-RNAi (BDSC, #57790; FBst0057790), UAS-Sce-RNAi (BDSC, #67924; FBst0067924), UAS-Ufd1-RNAi (BDSC, #41823; FBst0041823), UAS-CG8042-RNAi (BDSC, #41604; FBst0041604), UAS-Faf2-RNAi (BDSC, #43224; FBst0043224), UAS-casp-RNAi (BDSC, #44027; FBst0044027), UAS-Npl4-RNAi (BDSC, #53004; FBst0053004), UAS-elg1-RNAi (BDSC, #63551; FBst0063551), UAS-Rpt2-RNAi (BDSC, #34795; FBst0034795), Sce^KO (BDSC, #80157; FBst0080157), UAS-GFP^nls (lab stock), VasaGal4; hsFLP.D5, UAS-GFP/cyo; FRT82B, tubGal80/TM6B (Butsch et al., 2022 (link)), EyaGal4 (Leatherman and DiNardo, 2008 (link)), UASp-FRT-H2A-eGFP-PolyA-FRT-H2A-mCherry (Kahney et al., 2021 (link)), tubGal80^AID (McClure et al., 2022 (link)) (BDSC, #92470; FBst0092470) and UAS-VCP^K2A (Chang et al., 2021 (link)).

Previously described fly strains used in this work are 1: w¹¹¹⁸, 2: dhipk-Gal4 (hipk[BG00855], BDSC #12779), 3: UAS-GFP (BDSC #5431), 4: UAS-pc^RNAi (BDSC #33964), 5: UAS-e(z)^RNAi (BDSC #36068), 6: UAS-sce^RNAi (BDSC #67924), 7: UAS-ph-d^RNAi (BDSC #63018) 8: dhipk⁴ (Lee et al. 2009a (link)), 9: dpp-Gal4/TM6B (Staehling-Hampton et al. 1994 (link)), 10: UAS-HA-dhipk^attp40 (Tettweiler et al. 2019 (link)), 11: eyFLP; act>y⁺>Gal4, UAS-GFP (Pagliarini and Xu 2003 (link)). The details of how UAS-myc-hHIPK1^attp40, UAS-myc-hHIPK2^attp40, UAS-myc-hHIPK3^attp40, and UAS-myc-hHIPK4^attp40 were generated for this work is detailed in the section titled “Generation of plasmids and transgenic UAS-hHIPK fly stocks.” dhipk mutant rescue experiments were performed at 18°C and 25°C to determine the ideal Hipk expression levels by modulating the expression of Gal4-driven UAS-Hipk constructs, while experiments using dpp-Gal4 were performed at 29°C to increase UAS-Hipk expression. Flies were raised on standard media composed of 0.8 g agar, 2.3 g yeast, 5.7 g cornmeal, and 5.2 mL molasses per 100 ml. “BDSC” is an acronym for the Bloomington Drosophila Stock Center.